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Screening for DEL phenotype in RhD negative Indians.

Swati Kulkarni1, Disha S Parchure1, Vidya Gopalkrishnan1

  • 1Department of Transfusion Medicine, National Institute of Immunohaematology, Mumbai, India.

Journal of Clinical Laboratory Analysis
|June 24, 2017
PubMed
Summary

The DEL phenotype, a weak RhD variant, was investigated in Indian RhD-negative individuals. Two common DEL alleles, RHD(K409K) and RHD(M295I), were not detected, suggesting their rarity in this population.

Keywords:
D variantsDEL phenotypeIndiansRhD negativepolymerase chain reaction using sequence specific primers

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Area of Science:

  • Hematology
  • Genetics
  • Immunology

Background:

  • The DEL phenotype is a weak RhD variant, often misidentified as RhD-negative, potentially causing transfusion complications.
  • Molecular methods are crucial for identifying DEL variants, prevalent in East Asian and Caucasian populations.

Purpose of the Study:

  • To screen RhD-negative Indian individuals for the two most common DEL mutations: RHD(K409K) and RHD(M295I).
  • To assess the prevalence of these specific DEL alleles in the Indian population due to limited existing data.

Main Methods:

  • Serological testing for Rh antigens (C, c, D, E, e) and DEL phenotype confirmation using adsorption-elution.
  • Molecular analysis using Polymerase Chain Reaction-Sequence Specific Priming (PCR-SSP) to detect RHD(K409K) and RHD(M295I) alleles in 900 RhD-negative individuals.

Main Results:

  • Rh phenotyping confirmed 153 r'r, 10 r''r, and 737 rr phenotypes among the 900 participants.
  • All samples tested negative for RhD antigen by adsorption and elution.
  • Neither the RHD(K409K) nor the RHD(M295I) DEL alleles were detected in the study cohort.

Conclusions:

  • The two common DEL alleles screened are rare in the studied Indian RhD-negative population.
  • Further comprehensive studies with larger sample sizes are recommended to investigate other potential DEL mutations in India.