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Related Experiment Videos

Particulate fraction in amniotic fluid at second trimester.

H Jalanko, J Rapola, E Lehtonen

    Journal of Clinical Pathology
    |September 1, 1985
    PubMed
    Summary

    Microvillar enzymes in amniotic fluid, crucial for cystic fibrosis prenatal diagnosis, are primarily found in particulate fractions originating from the fetal intestine. This finding aids in understanding prenatal diagnostic markers.

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    Area of Science:

    • Biochemistry
    • Prenatal Diagnostics
    • Gastroenterology

    Background:

    • Microvillar enzymes in amniotic fluid are key markers for prenatal diagnosis of cystic fibrosis.
    • These enzymes are often found in particulate fractions within the amniotic fluid.

    Purpose of the Study:

    • To investigate the origin and characteristics of microvillar enzymes in amniotic fluid during mid-pregnancy.
    • To determine if these enzymes are associated with fetal intestinal components.

    Main Methods:

    • Sepharose 6B chromatography and ultracentrifugation to isolate enzyme-bound particulate fractions.
    • Electron microscopy to characterize the particulate fraction.
    • Sorbitol gradient centrifugation to assess heterogeneity.
    • Immunological assays (immunodiffusion, enzyme immunoassay, immunoperoxidase staining) to compare amniotic fluid and meconium fractions.

    Main Results:

    • Enzymes were predominantly bound to a particulate fraction (membrane fragments, vesicles) in amniotic fluid.
    • This fraction represented a small percentage (<1%) of total cell-free amniotic fluid protein.
    • Amniotic fluid and meconium particulate fractions exhibited similar enzyme patterns.
    • Antiserum against amniotic fluid particulate fraction cross-reacted with early meconium and fetal small intestine.

    Conclusions:

    • The enzyme-rich particulate fraction in mid-pregnancy amniotic fluid likely originates from the fetal intestine.
    • This provides insight into the source of prenatal diagnostic markers for cystic fibrosis.

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