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Effects of EF-24, RAD001, and paclitaxel on the expression profiles of apoptotic and anti-apoptotic genes

Ebru Alp1, Akin Yilmaz2, Hacer Ilke Onen3

  • 1Department of Medical Biology, Faculty of Medicine, Giresun University, Giresun, Turkey.

Journal of Cancer Research and Therapeutics
|June 24, 2017
PubMed
Abstract

Insights

This study investigated how paclitaxel, EF-24, and RAD001 affect apoptotic and anti-apoptotic gene expression in MDA-MB-231 breast cancer cells. Results showed differential transcriptional responses, highlighting the importance of understanding these changes for targeted cancer therapy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Cancer cells exhibit varied responses to chemotherapy and inhibitors.
  • Limited research exists on the effects of EF-24 and RAD001 on MDA-MB-231 breast cancer cells' apoptotic and anti-apoptotic gene expression.

Purpose of the Study:

  • To investigate mRNA expression levels of key apoptotic (CASP2, CASP8, CASP9) and anti-apoptotic (BCL2, BCL2L1) genes.
  • To analyze the impact of paclitaxel, EF-24, and RAD001 on these genes in MDA-MB-231 cells.

Main Methods:

  • Cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
  • Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to analyze mRNA expression levels.

Main Results:

  • A dose-dependent decrease in cell viability was observed for all tested compounds.
  • MDA-MB-231 cells demonstrated distinct transcriptional responses to paclitaxel, EF-24, and RAD001 concerning apoptotic and anti-apoptotic genes.

Conclusions:

  • The study found differential responses of MDA-MB-231 cells to paclitaxel, EF-24, and RAD001 at the transcriptional level of apoptosis-related genes.
  • Understanding these transcriptional changes is crucial for more accurate insights into apoptotic pathway inhibition and developing targeted cancer therapies.

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