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Comparisons between commercial salivary testosterone enzyme-linked immunosorbent assay kits
Christoffer R Andersson1, Jonas Bergquist2, Elvar Theodorsson3
1a Department of Neurology, Faculty of Medicine and Health , Örebro University , Örebro , Sweden.
Introduction:
Measuring testosterone concentrations is of interest both in clinical situations and for research, the latter expanding rapidly during recent years. An increased demand for convenient methods has prompted a number of companies to develop enzyme-linked immunosorbent assay (ELISA) kits to measure testosterone concentrations in saliva. However, the inter-comparability of kits from different manufacturers have yet to be determined.
Aim Of Study:
The aim of this study was to compare commercially available ELISA kits from four different manufacturers (Salimetrics, IBL, DRG and Demeditec).
Methods:
Saliva was collected from 50 participants (25 men and 25 women). Each sample was analysed by the four ELISA kits.
Results:
The correlations between the ELISA kits from Demeditec, DRG and Salimetrics were moderate to high with r-values > .77; however, proportional errors between the methods calls for caution. The ELISA kit from IBL malfunctioned and no results from this kit was obtained.
Conclusions:
Results from studies using the ELISA kits from Demeditec, DRG and Salimetrics are generally comparable; however, translation using the formulae presented in the current study could increase the accuracy of these comparisons.
