Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

1.7K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.7K
Photoluminescence: Applications01:14

Photoluminescence: Applications

1.2K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.2K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multimodal Fusion of Intraoperative FLIm and Preoperative PET/CT for Patient-Level Prediction of Lymph Node Metastasis in Head and Neck Cancer.

Cancers·2026
Same author

Fluorescence properties of collagen types I-V: a comprehensive study of spectral and lifetime characteristics.

Journal of biomedical optics·2026
Same author

Real-time fidelity assessment of fluorescence molecular imaging without reference images.

Journal of biomedical optics·2026
Same author

Slide-free, point-of-care imaging of core needle biopsies for rapid histologic assessment.

Biomedical optics express·2026
Same author

Fluorescence lifetime imaging of 5-ALA-induced PpIX and autofluorescence for detecting infiltrating glioblastoma margins in patients.

Biomedical optics express·2026
Same author

Fluorescence Properties of Collagen Types I-V: A Comprehensive Study of Spectral and Lifetime Characteristics.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Feb 27, 2026

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
10:41

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

9.1K

Rapid fluorescence lifetime estimation with modified phasor approach and Laguerre deconvolution: a comparative study.

Farzad Fereidouni1, Dimitris Gorpas, Dinglong Ma

  • 1Department of Pathology and Laboratory Medicine, 4400 V Street, CA 95817, United States of America.

Methods and Applications in Fluorescence
|June 24, 2017
PubMed
Summary

Real-time fluorescence lifetime analysis is key for medical imaging. This study compares the Phasor approach and Laguerre deconvolution for accurate, fast lifetime extraction from fluorescence decay curves.

More Related Videos

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

26.0K
Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
08:43

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

1.9K

Related Experiment Videos

Last Updated: Feb 27, 2026

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
10:41

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

9.1K
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

26.0K
Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
08:43

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

1.9K

Area of Science:

  • Biomedical optics
  • Fluorescence spectroscopy
  • Medical imaging technology

Background:

  • Fluorescence lifetime imaging (FLIM) is vital for biological tissue analysis at the mesoscopic level.
  • Real-time fluorescence decay analysis is essential for clinical applications like tumor margin delineation and atherosclerotic plaque imaging.

Purpose of the Study:

  • To compare the performance of two non-parametric, fit-free methods for real-time fluorescence lifetime determination.
  • To evaluate the Phasor approach and Laguerre deconvolution in terms of accuracy and speed.
  • To assess the influence of noise and model parameters on both methods.

Main Methods:

  • Utilized simulated and experimental fluorescence decay data.
  • Applied the Phasor approach for lifetime analysis.
  • Employed Laguerre deconvolution for lifetime analysis.
  • Compared accuracy, speed, and parameter dependence of both methods.

Main Results:

  • Both Phasor and Laguerre deconvolution methods provide real-time fluorescence lifetime values.
  • Performance comparison based on accuracy, speed, and sensitivity to noise and model parameters was conducted.
  • Quantitative results detailing the strengths and weaknesses of each method under various conditions were obtained.

Conclusions:

  • The Phasor approach and Laguerre deconvolution are viable non-parametric methods for real-time fluorescence lifetime analysis.
  • Understanding their comparative performance is crucial for selecting the optimal method for specific clinical applications.
  • Further optimization and validation are recommended for robust clinical translation of FLIM techniques.