Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Chromatin Packaging

Chromatin Packaging

Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter?
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...

Chromatin Packaging

Chromatin Packaging

Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...

The DNA Helix

The DNA Helix

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells.

Nature communications·2026

Same author

FET proteins and PARylation-dependent condensates promote replication fork reversal and genome stability.

Nature communications·2026

Same author

Condensin and topoisomerases cooperate to relieve topological stress at stalled replication forks.

Nature communications·2026

Same author

CDK4/6 inhibitor ribociclib and doxorubicin combination treatment inhibits breast cancer bone metastasis and enhances T-cell targeted therapy.

Journal of bone oncology·2026

Same author

The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells.

bioRxiv : the preprint server for biology·2026

Same author

Correction: GAS6/AXL Inhibition Enhances Ovarian Cancer Sensitivity to Chemotherapy and PARP Inhibition through Increased DNA Damage and Enhanced Replication Stress.

Molecular cancer research : MCR·2026

Same journal

Clinical Europium fluorescent based lectin assays for mucin O-glycomics.

Methods in enzymology·2026

Same journal

A dual-color FRET assay for detection and quantitative analysis of O-glycopeptidases.

Methods in enzymology·2026

Same journal

Evolutionary genetic approaches to analyze mucins.

Methods in enzymology·2026

Same journal

Ex vivo imaging and enzymatic analysis of intestinal mucus.

Methods in enzymology·2026

Same journal

Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues.

Methods in enzymology·2026

Same journal

Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ.

Methods in enzymology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 27, 2026

Demonstration of the DNA Fiber Assay for Investigating DNA Damage and Repair Dynamics Induced by Nanoparticles

Demonstration of the DNA Fiber Assay for Investigating DNA Damage and Repair Dynamics Induced by Nanoparticles

Published on: March 3, 2023

DNA Fiber Analysis: Mind the Gap!

Annabel Quinet¹, Denisse Carvajal-Maldonado¹, Delphine Lemacon¹

¹Saint Louis University School of Medicine, St. Louis, MO, United States.

Methods in Enzymology

|June 25, 2017

Summary

This summary is machine-generated.

The DNA fiber assay tracks DNA replication dynamics and stress responses. A modified assay detects single-stranded DNA gaps, crucial intermediates in DNA repair and cell survival mechanisms.

Keywords:

DNA fiber analysis DNA replication Postreplication repair Replication stress S1 nuclease Single-stranded DNA gap

More Related Videos

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

Published on: February 3, 2022

Related Experiment Videos

Last Updated: Feb 27, 2026

Demonstration of the DNA Fiber Assay for Investigating DNA Damage and Repair Dynamics Induced by Nanoparticles

Demonstration of the DNA Fiber Assay for Investigating DNA Damage and Repair Dynamics Induced by Nanoparticles

Published on: March 3, 2023

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

Published on: February 3, 2022

Area of Science:

Molecular Biology
Genetics
Cell Biology

Background:

Replication stress response is vital for cell survival and understanding human diseases.
The DNA fiber assay offers single-molecule resolution for studying replication fork dynamics.
Genotoxic agents perturb DNA replication, necessitating robust analytical tools.

Purpose of the Study:

To review DNA fiber preparation methods and their limitations.
To explore DNA fiber analysis for replication parameters like fork progression and origin firing.
To introduce a modified assay for detecting single-stranded DNA gaps during replication.

Main Methods:

Review of various DNA fiber preparation techniques.
Application of DNA fiber analysis to study replication fork dynamics.
Development of a modified DNA fiber protocol using ssDNA-specific endonuclease.

Main Results:

Standard DNA fiber assays have limitations in detecting single-stranded DNA gaps.
A novel strategy using endonuclease digestion allows detection of ssDNA gaps.
The modified assay enables study of post-replicative repair of ssDNA gaps.

Conclusions:

DNA fiber assays are powerful tools for studying DNA replication and stress responses.
Modified DNA fiber protocols enhance the detection of critical replication intermediates like ssDNA gaps.
These advancements aid in understanding cell survival mechanisms and disease pathologies.