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Collection, processing and freezing of equine bone marrow cells.
Fernanda Z Bastos1, Fernanda C M Barussi1, Thasla F Santi2
1Department of Animal Science, School of Life Sciences - Pontifícia Universidade Católica Do Paraná, Curitiba, Paraná, Brazil.
Cryobiology
|June 25, 2017
Summary
Collecting large volumes of equine bone marrow from older horses is safe. Bone marrow mononuclear cells (BMMCs) remain viable after cryopreservation, though recovery decreases with successive aspirations.
Area of Science:
- Veterinary Medicine
- Cell Biology
- Cryopreservation
Background:
- Equine bone marrow collection and processing lack standardized protocols.
- Advanced age in horses presents unique challenges for cell collection.
- Cryopreservation of equine bone marrow mononuclear cells (BMMCs) requires optimized methods.
Purpose of the Study:
- To describe large-volume bone marrow collection from aged horses.
- To assess bone marrow mononuclear cells (BMMCs) recovery and viability post-cryopreservation.
- To evaluate the impact of heparin concentration on cell aggregation and viability.
Main Methods:
- Bone marrow was collected from the sternums of 14 horses (aged 3-24 years).
- Syringes contained Iscove's modified Dulbecco's medium with varying sodium heparin concentrations.
- Bone marrow mononuclear cells (BMMCs) were quantified, viability assessed, and samples cryopreserved.
Main Results:
- Large-volume sternal bone marrow collection is safe in aged horses.
- Cell recovery significantly decreased with successive aspirations (p < 0.0001).
- Heparin concentration influenced cell aggregation, and cryopreserved BMMCs remained viable for over 150 days.
Conclusions:
- Sternal bone marrow collection is a feasible and safe method for obtaining large volumes from aged horses.
- Optimized heparin concentrations are crucial for minimizing cell aggregation during collection.
- Cryopreserved equine BMMCs maintain commercial viability, supporting their use in research and therapeutics.