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Related Experiment Video

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Microcrystal Electron Diffraction of Small Molecules
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Taking the measure of MicroED.

Jose A Rodriguez1, David S Eisenberg2, Tamir Gonen3

  • 1Department of Chemistry and Biochemistry, UCLA-DOE Institute, University of California, Los Angeles, Los Angeles, CA, USA.

Current Opinion in Structural Biology
|June 27, 2017
PubMed
Summary

Microcrystal electron diffraction (MicroED) now routinely determines atomic resolution structures from small protein crystals. This technique has advanced structural biology, enabling novel discoveries and offering broad opportunities for nanoscale crystallography.

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Area of Science:

  • Structural Biology
  • Biophysics
  • Crystallography

Background:

  • Electron cryo-microscopy (cryoEM) enables routine atomic resolution structure determination.
  • Microcrystal electron diffraction (MicroED), developed in 2013, is a key technique facilitating cryoEM.
  • MicroED has been used to determine structures of proteins across a wide molecular weight range.

Purpose of the Study:

  • To summarize key learnings from the initial years of MicroED application.
  • To re-evaluate theoretical aspects of crystal selection and data extraction for MicroED.
  • To explore the future potential of MicroED in structural biology over the next decade.

Main Methods:

  • Application of MicroED to various protein crystals (size, shape, symmetry, solvent content).
  • Analysis of structural data obtained from MicroED.
  • Theoretical evaluation of MicroED methodology.

Main Results:

  • MicroED has successfully determined protein structures with resolutions from 1.0Å to 3.2Å (mostly <2.5Å).
  • The method is effective for a diverse range of crystal types, including nanocrystals.
  • The first ab initio structure determination using MicroED was achieved.

Conclusions:

  • MicroED is a powerful and versatile tool for atomic resolution structure determination.
  • Ongoing advancements in MicroED offer significant opportunities for nanocrystal structure analysis.
  • The technique is poised for widespread adoption and further innovation in the coming decade.