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Related Concept Videos

Proteomics01:33

Proteomics

10.0K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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Related Experiment Video

Updated: Feb 27, 2026

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
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Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

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Pattern of Protein Expression in Developing Wheat Grains Identified through Proteomic Analysis.

Mingming Yang1,2, Xiang Gao1,2, Jian Dong1,2

  • 1State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F UniversityYangling, China.

Frontiers in Plant Science
|June 27, 2017
PubMed
Summary

Understanding early grain development in wheat is key to improving crop yield. This study identified key proteins and genomic regions, like chromosome 4D, influencing grain size and development.

Keywords:
chromosomegrain developmentmetabolic pathwayproteomewheat

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Area of Science:

  • Plant Biology
  • Proteomics
  • Genetics

Background:

  • Grain development significantly impacts final crop yield in wheat.
  • Understanding molecular mechanisms during early grain development is crucial for yield improvement.

Purpose of the Study:

  • To profile and analyze proteomes during early grain development in two common wheat cultivars with contrasting grain sizes.
  • To identify differentially expressed proteins (DEPs) and understand their roles in grain development.

Main Methods:

  • Utilized isobaric tags for relative and absolute quantitation (iTRAQ) based proteomics.
  • Compared proteomes of wheat cultivars P271 (large grains) and Chinese Spring (CS) (small grains) at three developmental stages.
  • Performed bioinformatic analyses and in silico chromosome localization of DEPs.

Main Results:

  • Over 3,600 proteins accumulated during early grain development in both cultivars.
  • Identified 130 DEPs between cultivars and 306 proteins with developmental stage-specific accumulation.
  • Bioinformatic analysis revealed developmental differences and highlighted chromosome 4D's significant role in protein distribution.

Conclusions:

  • Differential protein expression patterns provide insights into molecular processes governing grain size differences.
  • Chromosome 4D harbors key genetic factors influencing grain yield traits, consistent with previous quantitative trait locus (QTL) studies.
  • This research offers valuable molecular insights for enhancing wheat grain yield.