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Characterization of the phosphate-specific transport system in Cronobacter sakazakii BAA-894
X Liang1,2, X Hu1,3, X Wang1,3
1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.
Aims:
Characterize the phosphate-specific transport system in Cronobacter sakazakii BAA-894.
Methods And Results:
The genes relevant to phosphate transfer in C. sakazakii BAA-894 were determined by using sequence alignment to the corresponding genes in Escherichia coli. Then, the determined pst operon in C. sakazakii BAA-894 was deleted using the lambda Red recombination system. Using the wild type C. sakazakii BAA-894 as a control, the membrane permeability, auto-aggregation, exopolysaccharide biosynthesis, biofilm formation, and adhesion ability of the mutant ▵pst grown in media containing high or low concentrations of phosphate were investigated; stronger auto-aggregation, less biofilm formation and higher adhesion ability were observed in ▵pst cells grown in low phosphate media. Transcriptome analysis showed that phosphate availability has a global influence to C. sakazakii BAA-894 and ▵pst cells.
Conclusions:
Phosphorus availability is important for C. sakazakii in many ways including biofilm formation and adhesion ability.
Significance And Impact Of The Study:
This study demonstrates that phosphate availability has a global influence to C. sakazakii, expends our understanding to the phosphate transfer in C. sakazakii, and is helpful for revealing the survival mechanism of C. sakazakii under stress conditions.
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