Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to the Cytoskeleton01:33

Introduction to the Cytoskeleton

37.6K
Overview of the Cytoskeleton
The cytoskeleton is a network of protein filaments present within the cell, having three distinct filaments ̶   microfilaments, microtubules, and intermediate filaments. Each has characteristic features that distinguish them, including the dynamics of their assembly and disassembly, mechanical properties, polarity, and the type of molecular motors associated with them. Earlier, they were thought to be present only in eukaryotic cells; however, their...
37.6K
Adaptability of Cytoskeletal Filaments01:12

Adaptability of Cytoskeletal Filaments

6.2K
The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...
6.2K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

10.2K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.2K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

3.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.3K
Cytoplasm01:24

Cytoplasm

8.6K
The cytoplasm consists of organelles and a framework of protein scaffolds called the cytoskeleton suspended in an aqueous solution, the cytosol. The cytosol is a rich broth of water, ions, salts, and various organic molecules.
Protein Folding and Misfolding
The cytoplasm is the location for several cellular processes, including protein synthesis and folding. The aqueous nature of the cytosol promotes protein folding such that the hydrophobic amino acid side chains are buried in the protein...
8.6K
Cytoplasm01:16

Cytoplasm

92.9K
The cytoplasm consists of organelles and a framework of protein scaffolds called the cytoskeleton suspended in an aqueous solution, the cytosol. The cytosol is a rich broth of water, ions, salts, and various organic molecules.
Protein Folding and Misfolding
The cytoplasm is the location for several cellular processes, including protein synthesis and folding. The aqueous nature of the cytosol promotes protein folding such that the hydrophobic amino acid side chains are buried in the protein...
92.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interferon Regulatory Factor Family Transcription Factor Expression in the Dorsal Root Ganglion of a Rat Model of Neuropathic Pain.

Journal of Nippon Medical School = Nippon Ika Daigaku zasshi·2026
Same author

Pretreatment serum vitamin D levels predict the therapeutic efficacy of atezolizumab plus bevacizumab therapy in advanced hepatocellular carcinoma: a multicenter analysis.

International journal of clinical oncology·2026
Same author

Programming Nonlinear Interfacial Mechanics of Synthetic Cells: Lipid Geometry and DNA Nanostructures.

Small science·2026
Same author

Reelin level in serum-derived extracellular vesicles predicts regression of M2BPGi-defined liver fibrosis following hepatitis C virus eradication by direct-acting antiviral agents.

Journal of gastroenterology·2026
Same author

Non-diffusive slow heat dissipation induces high local temperature in living cells.

Nature communications·2026
Same author

Being Overweight or Obese Contributes to a Deterioration of the Liver Function in Steatotic Liver Disease: A Long-term Follow-up of Health Check-up Participants.

Internal medicine (Tokyo, Japan)·2026

Related Experiment Video

Updated: Feb 27, 2026

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold
07:09

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Published on: October 26, 2018

6.6K

DNA cytoskeleton for stabilizing artificial cells.

Chikako Kurokawa1, Kei Fujiwara2, Masamune Morita3

  • 1Department of Applied Physics, Tokyo University of Agriculture and Technology, Tokyo 184-8588 Japan.

Proceedings of the National Academy of Sciences of the United States of America
|June 28, 2017
PubMed
Summary

Researchers created a robust artificial cytoskeleton using DNA nanostructures to stabilize cell-sized lipid droplets and liposomes, enhancing their potential for biomedical applications.

Keywords:
DNA gelcytoskeletonlipid dropletliposomeself-assembly

More Related Videos

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures
08:02

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

1.5K
Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

7.2K

Related Experiment Videos

Last Updated: Feb 27, 2026

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold
07:09

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Published on: October 26, 2018

6.6K
Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures
08:02

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

1.5K
Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

7.2K

Area of Science:

  • Biomaterials Science
  • Synthetic Biology
  • Nanotechnology

Background:

  • Cell-sized liposomes and droplets are fragile platforms for artificial cells and biomedical tools due to the lack of cytoskeletons.
  • Existing systems lack the structural integrity required for advanced applications.

Purpose of the Study:

  • To engineer a stabilizing artificial cytoskeleton for lipid-based cell mimics.
  • To enhance the mechanical stability and resilience of liposomes and droplets.

Main Methods:

  • Constructed Y-shaped DNA nanostructures that self-assemble into networks.
  • Utilized cationic lipids to form an undercoat of DNA networks beneath lipid membranes.
  • Encapsulated DNA structures within droplets to create a stabilizing DNA gel shell.

Main Results:

  • The DNA gel shell significantly increased interfacial tension, elastic modulus, and shear modulus of the droplet surface.
  • Liposomes with DNA gel shells exhibited enhanced tolerance to osmotic shock.
  • Stability improvements were observed when the DNA shell was in the gel phase.

Conclusions:

  • DNA nanostructures can form an artificial cytoskeleton, stabilizing lipid membranes.
  • This DNA gel shell mimics the stabilizing role of a cytoskeleton in live cells.
  • The engineered system offers a promising platform for robust artificial cells and biomedical tools.