Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In-space manufacturing of optical lenses: Fluidic Shaping aboard the International Space Station.

NPJ microgravity·2026
Same author

Magnetothermally responsive fibrin hydrogel for localized neuromodulation.

Colloids and surfaces. B, Biointerfaces·2026
Same author

Modeling the thermal behavior of photopolymers for in-space fabrication.

NPJ microgravity·2026
Same author

Dual-Frequency Ultrasound Enhances Cavitation of Microdroplets for Controlled Scaffold Porosity in Tissue Engineering.

ACS applied materials & interfaces·2026
Same author

Nanoparticles-Mediated Modulation of TRP Channels: Advances and Therapeutic Potential.

Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology·2026
Same author

TYRAY-Functionalized Alginate Bioinks for 3D Bioprinting Support Stem Cell Culture and Endothelial Network Formation.

ACS biomaterials science & engineering·2025

Related Experiment Video

Updated: Feb 27, 2026

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

3.4K

Rapid phenotypic antimicrobial susceptibility testing using nanoliter arrays.

Jonathan Avesar1, Dekel Rosenfeld1, Marianna Truman-Rosentsvit2

  • 1Faculty of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa 3200003, Israel.

Proceedings of the National Academy of Sciences of the United States of America
|June 28, 2017
PubMed
Summary

Rapid antibiotic susceptibility testing using nanoliter droplet arrays offers a faster alternative to conventional methods. This innovation aids in the timely and effective use of antibiotics in clinical settings.

Keywords:
antibiotic resistanceantibiotic susceptibility testingmicrofluidicsnanoliter wellsresazurin

More Related Videos

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
07:35

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

13.3K
High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
11:00

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

12.5K

Related Experiment Videos

Last Updated: Feb 27, 2026

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

3.4K
Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
07:35

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

13.3K
High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
11:00

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

12.5K

Area of Science:

  • Microbiology
  • Biotechnology
  • Clinical Diagnostics

Background:

  • Antibiotic resistance is a significant global health challenge.
  • Effective antibiotic use necessitates rapid antimicrobial susceptibility testing (AST) in clinical settings.
  • Current AST methods can be time-consuming, delaying clinical decisions.

Purpose of the Study:

  • To develop a rapid and scalable method for AST.
  • To reduce the time required for AST to enable same-day clinical decisions.
  • To enhance the practicality and translatability of AST for clinical use.

Main Methods:

  • Utilized stationary nanoliter droplet arrays for AST.
  • Developed an algorithm for automated data analysis.
  • Implemented a multiplexing system for improved practicality.

Main Results:

  • Achieved AST results in approximately half the time of conventional methods.
  • Demonstrated a 2-day reduction in diagnostic time for bacteria from urine samples.
  • Validated the approach on numerous clinical isolates.

Conclusions:

  • The developed nanoliter droplet array method provides rapid and scalable AST.
  • This approach significantly reduces diagnostic time, facilitating same-day clinical application.
  • The system shows promise for improving antibiotic stewardship in healthcare settings.