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Related Experiment Video

Updated: Feb 27, 2026

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior
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Microfluidic guillotine for single-cell wound repair studies.

Lucas R Blauch1, Ya Gai1, Jian Wei Khor1

  • 1Department of Mechanical Engineering, Stanford University, Stanford, CA 94305.

Proceedings of the National Academy of Sciences of the United States of America
|June 28, 2017
PubMed
Summary

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Molecular pathways for learning in the single-cell Stentor coeruleus.

Current biology : CB·2026

Researchers developed a microfluidic guillotine to rapidly wound single cells, enabling high-throughput studies of cell repair mechanisms. This new method significantly advances our understanding of cellular regeneration and viability.

Area of Science:

  • Cellular Biology
  • Biophysics
  • Regenerative Medicine

Background:

  • Wound repair is a fundamental characteristic of life.
  • Single-cell wound healing is an emerging research area.
  • Existing methods for inducing cell damage lack reproducibility and throughput.

Purpose of the Study:

  • To develop a novel, high-throughput method for wounding single cells.
  • To investigate the dynamics and viability of cells undergoing repair after bisection.
  • To enable time-course mechanistic studies of single-cell wound repair.

Main Methods:

  • A microfluidic guillotine was designed to bisect single Stentor coeruleus cells.
  • Continuous-flow processing allowed for high-throughput cell manipulation.
Keywords:
Stentor coeruleusmicrofluidicsmicroguillotinesingle cellwound healing

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  • Local cutting dynamics were analyzed under varying viscous stress conditions.

    • Main Results:

    • Two distinct bisection regimes were identified based on viscous stress and membrane rupture.
    • High throughput of up to 64 cells per minute was achieved, over 200x faster than current methods.
    • Over 100 cells were synchronized in their repair process, facilitating mechanistic studies.

    Conclusions:

    • The microfluidic guillotine provides a reproducible and high-throughput method for single-cell wounding.
    • This technique significantly advances the study of cellular repair mechanisms and viability.
    • The method enables unprecedented time-course mechanistic investigations in single-cell biology.