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Oxidative DNA damage during night shift work.

Parveen Bhatti1, Dana K Mirick1, Timothy W Randolph2

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|June 28, 2017
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Summary
This summary is machine-generated.

Shift work, particularly night work, is linked to lower levels of DNA repair marker 8-hydroxydeoxyguanosine (8-OH-dG). This reduction is likely due to suppressed melatonin, impacting DNA repair capacity in shift workers.

Keywords:
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Area of Science:

  • Occupational Health
  • Molecular Biology
  • Chronobiology

Background:

  • Shift work disrupts circadian rhythms and has been linked to health issues.
  • Previous research indicated reduced DNA repair during day sleep for shift workers, attributed to melatonin absence.
  • The role of night work in DNA repair and its association with melatonin levels remained unclear.

Purpose of the Study:

  • To investigate the association between night work and urinary 8-hydroxydeoxyguanosine (8-OH-dG) excretion.
  • To determine if melatonin suppression during night work contributes to reduced DNA repair.
  • To compare DNA repair markers during night work versus night sleep in shift workers.

Main Methods:

  • A cross-sectional study involving 50 shift workers selected based on melatonin level differences.
  • Measurement of urinary 6-sulfatoxymelatonin and 8-OH-dG concentrations.
  • Analysis using mixed effects models to compare 8-OH-dG levels during night work and night sleep.

Main Results:

  • Circulating melatonin levels were significantly lower during night work compared to night sleep.
  • Urinary 8-OH-dG levels during night work were substantially reduced, approximately 20% of night sleep levels.
  • A statistically significant association (p<0.001) was found between night work and reduced 8-OH-dG excretion.

Conclusions:

  • Night work is associated with reduced repair of oxidative DNA damage (8-OH-dG lesions) compared to night sleep.
  • Melatonin suppression during night work is the likely driver of this reduced DNA repair capacity.
  • Future research should explore melatonin supplementation to enhance DNA repair in shift workers.