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SUN1 silencing inhibits cell growth through G0/G1 phase arrest in lung adenocarcinoma

Weiyi Huang1, Haihua Huang1, Lei Wang1

  • 1Department of Oncology, The First People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, People's Republic of China.

Oncotargets and Therapy
|June 28, 2017
PubMed
Abstract

Insights

Sad-1 and UNC-84 domain containing 1 (SUN1) is crucial for lung adenocarcinoma cell growth. Silencing SUN1 inhibits proliferation and causes cell cycle arrest, indicating its potential as a therapeutic target.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Oncology

Background:

  • The cytoskeleton is vital for carcinoma cell functions.
  • Sad-1 and UNC-84 domain containing 1 (SUN1) links the nucleoskeleton and cytoskeleton.
  • The role of SUN1 in lung adenocarcinoma remains largely unexplored.

Purpose of the Study:

  • To investigate the function of SUN1 in lung adenocarcinoma.
  • To determine SUN1's impact on cell proliferation, cell cycle, and related protein expression.

Main Methods:

  • Utilized lentivirus-mediated short hairpin RNA (shRNA) to silence SUN1 in lung adenocarcinoma cell lines (A549 and 95D).
  • Quantified SUN1 mRNA and protein levels using qRT-PCR and Western blotting.
  • Assessed cell proliferation (MTT assay), colony formation, and cell cycle distribution (flow cytometry).

Main Results:

  • SUN1 expression was significantly reduced at both mRNA and protein levels post-silencing.
  • SUN1 silencing markedly decreased cell proliferation and colony formation.
  • Suppression of SUN1 induced G0/G1 phase cell cycle arrest and reduced Cyclin D1, CDK6, and CDK2 expression.

Conclusions:

  • SUN1 plays a critical role in the proliferation of lung adenocarcinoma cells in vitro.
  • SUN1 represents a potential therapeutic target for lung adenocarcinoma treatment.
Keywords:
SUN1lung cancerproliferation

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