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Updated: Feb 27, 2026

Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound
Published on: March 4, 2015
Ultrasound Needle Visibility in Contrast Mode Imaging: An In Vitro and Ex Vivo Study
Marga B Rominger1, Katharina Martini1, Evelyn Dappa1,2,3
1University Hospital Zurich, University of Zurich, Institute of Diagnostic and Interventional Radiology, Zurich, Switzerland.
Purpose:
To evaluate needle visibility in ultrasound under contrast mode conditions.
Materials And Methods:
Needle visibility was evaluated for bevel, EchoTip ® and shaft of 18G Chiba biopsy needle with a 9 MHz linear probe (GE Logiq E9). Insertion angles varied between 30°(steep) and 90°(parallel to the probe surface). The acoustic output varied from 5-28%. 2 different contrast mode presets with either 'Amplitude Modulation' (Penetration) or 'Phase Inversion Harmonics' (High Resolution) were assessed. All other imaging parameters were kept constant. The visibility of bevel, EchoTip ® and shaft was assessed for grayscale and color-coded images with a 3-point Likert-like scale (not, poorly, well visible) by 2 independent readers. The echogenicity of the needle bevel, EchoTip ® and shaft was assessed in deciBel (dB) on the color-coded images.
Results:
With the parallel insertion angle, all needle areas were well visible. With steep insertion the EchoTip ® was the only visible area. High Resolution was superior to Penetration (p<0.001). The visibility and echogenicity of the needle bevel ( rgrayscale =0.109, p grayscale =0.178; rcolor-coded =0.236, p color-coded =0.266; rdB =0.956, p dB =0.001), EchoTip ® ( rgrayscale += 0.477, p grayscale += 0.018; rcolor-coded =0.540, p color-coded += 0.006; rdB =0.911, p dB =0.001) and shaft ( rgrayscale =0.124, p grayscale =0.563; rcolor-coded =0.061, p color-coded += 0.775; rdB += 0.926, p dB =0.001) increased with increasing acoustic output. Grayscale images were superior to color-coded images for needle visibility (p=0.004).
Conclusion:
Parallel needle insertion, use of an echogenic tip, adequate choice of presets, increased acoustic output, and dual view of grayscale and color-coded images improve needle visibility in ultrasound under contrast mode conditions.
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