Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Extrasensory Perception01:23

Extrasensory Perception

1.0K
Extrasensory perception, or ESP, suggests the ability to perceive events beyond the conventional senses of sight, hearing, and touch. Parapsychologists, who research ESP and related psychic phenomena, categorize ESP into three main types: precognition, telepathy, and clairvoyance.
Precognition involves foreseeing future events, such as predicting an accident before it happens. An example of precognition could be someone dreaming about a specific event, like a car crash, which then occurs...
1.0K
Photosystem I01:27

Photosystem I

70.6K
Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor.
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
70.6K
Defining Psychology01:24

Defining Psychology

8.2K
Psychology is the scientific discipline dedicated to understanding both observable behavior and the internal mental processes underlying such behavior. It aims to comprehend human nature and apply this understanding to solve practical problems, enhance well-being, and improve societal outcomes. An example of psychology's application is the study of prosocial behavior, such as why and under what conditions individuals might help strangers in need. This process involves describing observed...
8.2K
The Scope of Physics01:17

The Scope of Physics

54.2K
Physics is concerned with the interactions of energy, matter, space, and time, in order to discover the underlying mechanisms that underpin all phenomena. The word "physics" comes from the Greek word "phúsis", which means nature. Physics seeks to comprehend the natural world around us at its most fundamental level. It emphasizes the use of quantitative laws to do this, which could be valuable in other fields that want to push the performance boundaries of present...
54.2K
Psychology as a Science01:13

Psychology as a Science

4.1K
Psychology, as a scientific discipline, aims to understand the mind and behavior through rigorous and systematic methods. The foundation of psychological research is evidence-based, relying heavily on the scientific method to derive and validate knowledge. This structured approach ensures that findings are reliable, valid, and applicable to broader contexts.
The scientific method in psychology involves six critical steps: making observations, formulating hypotheses, conducting tests, analyzing...
4.1K
Application of Pascal's Law01:03

Application of Pascal's Law

11.1K
Pascal's experimentally proven observations—that a change in pressure applied to an enclosed fluid is transmitted undiminished throughout the fluid and to the walls of its container—provide the foundations for hydraulics, one of the most important developments in modern mechanical technology.
Hydraulic systems are used to operate automotive brakes, hydraulic jacks, and numerous other mechanical systems. We can derive a relationship between the forces in a simple hydraulic system...
11.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evolving concepts of the protein universe.

iScience·2025
Same author

Protein synthesis and its control in yeasts.

Yeast (Chichester, England)·2018
Same author

Guidelines and recommendations on yeast cell death nomenclature.

Microbial cell (Graz, Austria)·2018
Same author

Cellular factors important for the de novo formation of yeast prions.

Biochemical Society transactions·2008
Same author

Analysis of the generation and segregation of propagons: entities that propagate the [PSI+] prion in yeast.

Genetics·2003
Same author

The [URE3] phenotype: evidence for a soluble prion in yeast.

EMBO reports·2001

Related Experiment Video

Updated: Feb 27, 2026

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues
07:10

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues

Published on: February 3, 2023

1.7K

The life of [PSI].

Brian Cox1,2, Mick Tuite3

  • 1Kent Fungal Group, School of Biosciences, University of Kent, Canterbury, Kent, CT2 7NJ, UK. bsc1@kent.ac.uk.

Current Genetics
|June 28, 2017
PubMed
Summary
This summary is machine-generated.

The yeast Hsp104 protein manages prion inheritance through two distinct pathways, impacting protein disaggregation and cellular aging. These pathways are crucial for maintaining the [PSI+] prion and may indicate Hsp104

Keywords:
Hsp104PrionSporulationSup35Yeast[PSI]

More Related Videos

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics
13:16

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics

Published on: July 31, 2021

2.5K
A Tactile Automated Passive-Finger Stimulator TAPS
19:44

A Tactile Automated Passive-Finger Stimulator TAPS

Published on: June 3, 2009

14.2K

Related Experiment Videos

Last Updated: Feb 27, 2026

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues
07:10

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues

Published on: February 3, 2023

1.7K
Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics
13:16

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics

Published on: July 31, 2021

2.5K
A Tactile Automated Passive-Finger Stimulator TAPS
19:44

A Tactile Automated Passive-Finger Stimulator TAPS

Published on: June 3, 2009

14.2K

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Protein Homeostasis

Background:

  • The AAA+ disaggregase Hsp104 is vital for yeast prion maintenance and inheritance.
  • The [PSI+] prion, a form of the Sup35 protein, is uniquely managed by Hsp104 through two distinct pathways.
  • These pathways are implicated in cellular aging and protein quality control.

Purpose of the Study:

  • To investigate the dual roles of Hsp104 in [PSI+] prion inheritance in Saccharomyces cerevisiae.
  • To explore the connection between Hsp104's prion management functions and cellular aging processes.
  • To determine if Hsp104's role in prion management during sporulation indicates a function in cellular rejuvenation.

Main Methods:

  • Analysis of Hsp104 and Sup35 mutations affecting prion inheritance.
  • Manipulation of Hsp104 enzyme activity and expression levels.
  • Observation of prion inheritance during vegetative division and sporulation.

Main Results:

  • Hsp104 influences [PSI+] prion inheritance via two co-chaperone-dependent pathways.
  • One pathway involves protein disaggregation, the other involves retention of damaged proteins in the mother cell.
  • Mutations and manipulations affecting Hsp104 and Sup35 impact these pathways differently.
  • Hsp104 activity and expression changes affect prion inheritance in both budding and sporulation.

Conclusions:

  • Hsp104 employs distinct mechanisms to manage the [PSI+] prion, influencing protein quality control and aging.
  • The management of Sup35 prions during sporulation by Hsp104 may serve as a marker for its role in rejuvenation.
  • Further research into these pathways could elucidate Hsp104's broader role in cellular maintenance and longevity.