Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Editing02:23

RNA Editing

10.0K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
10.0K
CRISPR01:59

CRISPR

58.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.3K
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

9.6K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
9.6K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.7K
Overview
80.7K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

17.2K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
17.2K
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

4.9K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gonadal sex reversal at single-cell resolution in Znrf3-deficient mice.

Development (Cambridge, England)·2024
Same author

Preclinical research (on rare diseases): we need to talk about health equity.

Mammalian genome : official journal of the International Mammalian Genome Society·2024
Same author

SEC31A may be associated with pituitary hormone deficiency and gonadal dysgenesis.

Endocrine·2024
Same author

Origin, specification and differentiation of a rare supporting-like lineage in the developing mouse gonad.

Science advances·2022
Same author

Gadd45g is required for timely Sry expression independently of RSPO1 activity.

Reproduction (Cambridge, England)·2022
Same author

Homozygosity for a novel INHA mutation in two male siblings with hypospadias, primary hypogonadism, and high-normal testicular volume.

European journal of endocrinology·2022

Related Experiment Video

Updated: Feb 27, 2026

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
06:46

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

10.5K

Editing mammalian genomes: ethical considerations.

Andy Greenfield1

  • 1Mammalian Genetics Unit, MRC Harwell Institute, Harwell Campus, Didcot, Oxfordshire, OX11 0RD, UK. a.greenfield@har.mrc.ac.uk.

Mammalian Genome : Official Journal of the International Mammalian Genome Society
|June 28, 2017
PubMed
Summary

Genome editing allows unprecedented genome manipulation, revolutionizing gene function studies and disease modeling in mammals. This technology raises significant ethical questions regarding research practices and the 3Rs principles.

More Related Videos

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

23.3K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

36.0K

Related Experiment Videos

Last Updated: Feb 27, 2026

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
06:46

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

10.5K
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

23.3K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

36.0K

Area of Science:

  • Biotechnology
  • Genetics
  • Bioethics

Background:

  • Genome editing technologies enable large-scale genetic manipulation.
  • These tools offer revolutionary potential for studying gene function.
  • They are crucial for developing mammalian models of human genetic diseases.

Purpose of the Study:

  • To explore the disruptive potential of genome editing in research practices.
  • To assess the impact of genome editing on the ethical implementation of the 3Rs (Replacement, Reduction, Refinement).
  • To identify and discuss ethical questions arising from mammalian genome editing in biomedical research.

Main Methods:

  • Literature review of genome editing applications in biomedical research.
  • Ethical analysis framework applied to the 3Rs principles.
  • Discussion of case studies and potential future scenarios.

Main Results:

  • Genome editing significantly alters research methodologies and model generation.
  • The technology necessitates a re-evaluation of ethical considerations for animal research.
  • Specific ethical challenges include off-target effects, germline editing, and animal welfare.

Conclusions:

  • Genome editing presents both transformative opportunities and ethical challenges in biomedical research.
  • Adherence to and reinterpretation of the 3Rs are critical for responsible innovation.
  • Ongoing ethical discourse is essential for navigating the implications of genome editing in mammals.