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Protein Modifications in the RER

Protein Modifications in the RER

Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...

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Updated: Feb 27, 2026

Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry

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Published on: June 15, 2021

Residue-Specific Peptide Modification: A Chemist's Guide.

Justine N deGruyter¹, Lara R Malins¹, Phil S Baran¹

¹Department of Chemistry, The Scripps Research Institute , 10550 North Torrey Pines Road, La Jolla, California 92037, United States.

|June 28, 2017

Summary

This summary is machine-generated.

This guide helps researchers choose chemical methods for protein modification. It highlights advances in bioconjugation and native protein alteration over the last decade.

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Area of Science:

Chemical Biology
Biochemistry
Molecular Biology

Background:

Rapid advancements in bioconjugation and native protein modification necessitate efficient method selection.
Identifying optimal chemical strategies for specific amino acid residues in complex biological systems is challenging.

Purpose of the Study:

To provide a comprehensive manual on recent progress in chemical protein modification.
To assist researchers in selecting appropriate methods for peptide alteration.
To serve as a foundational resource for addressing persistent challenges in the field.

Main Methods:

Review of significant advances in bioconjugation techniques.
Analysis of native protein modification strategies.
Graphical representation of chemical methods for peptide alteration.

Main Results:

A curated overview of key developments in protein modification over the past ten years.
A framework for triaging candidate methods based on specific requirements.
Identification of promising approaches for future research.

Conclusions:

The manual offers a valuable resource for navigating the complexities of chemical protein modification.
It empowers researchers to make informed decisions, accelerating scientific discovery.
This work facilitates the resolution of long-standing challenges in bioconjugation and native protein alteration.