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Related Experiment Video

Updated: Feb 27, 2026

Video Tracking Protocol to Screen Deterrent Chemistries for Honey Bees
10:19

Video Tracking Protocol to Screen Deterrent Chemistries for Honey Bees

Published on: June 12, 2017

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Video Tracking Protocol to Screen Deterrent Chemistries for Honey Bees.

Nicholas R Larson1, Troy D Anderson2

  • 1Entomology, Virginia Tech; nlarson@vt.edu.

Journal of Visualized Experiments : Jove
|June 28, 2017
PubMed
Summary

New repellent chemicals can deter honey bees (Apis mellifera L.) from pesticide-treated crops. This study developed a method to test repellent effectiveness, showing honey bees avoid treated food sources.

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Area of Science:

  • Agricultural Science
  • Entomology
  • Environmental Science

Background:

  • Honey bees (Apis mellifera L.) are vital pollinators facing population declines.
  • Pesticide exposure is a significant factor contributing to honey bee colony losses.
  • New strategies are needed to mitigate pesticide risks to pollinators.

Purpose of the Study:

  • To develop and validate a protocol for assessing the efficacy of repellent chemistries against honey bees.
  • To determine if specific compounds can deter honey bees from potential exposure sites.

Main Methods:

  • Honey bee foragers were collected and starved prior to testing.
  • Individual bees were placed in arenas with control (sugar-agarose) or repellent-treated cubes.
  • Locomotor activity was recorded using video tracking software over a 10-minute period.

Main Results:

  • Honey bees demonstrated avoidance behavior towards sugar-agarose cubes containing repellent compounds.
  • Conversely, honey bees were attracted to control cubes without added compounds.
  • The developed protocol successfully differentiated between repellent and non-repellent treatments.

Conclusions:

  • Repellent chemistries show promise in deterring honey bees from recently treated crops.
  • This method provides a reliable way to screen potential repellents for pollinator protection.
  • Further research can refine these repellents for practical agricultural application.