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Updated: Feb 27, 2026

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Published on: June 15, 2017

Protocols for C-Brick DNA Standard Assembly Using Cpf1.

Shi-Yuan Li¹, Guo-Ping Zhao¹, Jin Wang²

¹Institute of Plant Physiology and Ecology, Shanghai Insititutes for Biological Sciences, Chinese Academy of Sciences.

Journal of Visualized Experiments : Jove

|June 28, 2017

Summary

This summary is machine-generated.

CRISPR-associated protein Cpf1 enables a new DNA assembly standard, C-Brick, for efficient gene part construction. This method offers long recognition sites and short scars, facilitating iterative DNA assembly for researchers.

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Last Updated: Feb 27, 2026

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Published on: June 15, 2017

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

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Author Spotlight: Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Author Spotlight: Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Published on: May 12, 2023

Area of Science:

Molecular Biology
Synthetic Biology
Biotechnology

Background:

CRISPR-associated protein Cpf1 (Cpf1) is an RNA-guided endonuclease that cleaves double-stranded DNA, producing sticky ends.
Existing DNA assembly standards like BioBrick and BglBrick have limitations that can be overcome with novel approaches.

Purpose of the Study:

To establish and describe a new DNA assembly standard, C-Brick, utilizing the unique properties of Cpf1.
To provide a detailed procedure for assembling DNA parts using the C-Brick standard.

Main Methods:

Utilizing Cpf1's ability to cleave DNA at specific recognition sites (T1-T4) on a C-Brick vector.
Employing complementary sticky ends generated by T2 and T3 site cleavage for DNA part assembly.
Leveraging the iterative nature of Cpf1 cleavage for sequential assembly of multiple DNA parts.

Main Results:

The C-Brick standard facilitates DNA assembly with long recognition sites and short "GGATCC" scars.
The assembly process is iterative, allowing for the construction of complex DNA constructs.
A clear procedure for C-Brick DNA assembly is detailed.

Conclusions:

The C-Brick standard offers an efficient and versatile method for DNA assembly.
This standard is accessible and applicable to a wide range of users, including scientists, students, and amateurs.
C-Brick has the potential to become a widely adopted standard in molecular biology and synthetic biology research.