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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

3.2K
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Affinity and Avidity01:41

Affinity and Avidity

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Overview
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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Ligand Binding and Linkage00:49

Ligand Binding and Linkage

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Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
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Updated: Feb 27, 2026

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
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Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

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Affimer proteins are versatile and renewable affinity reagents.

Christian Tiede1,2, Robert Bedford1,2, Sophie J Heseltine1,2

  • 1School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom.

Elife
|June 28, 2017
PubMed
Summary
This summary is machine-generated.

Affimer proteins offer a valuable alternative to antibodies for biological research. These versatile binding reagents can be used for protein studies, function modulation, and advanced imaging techniques.

Keywords:
AffimerE. coliantibodyassaybiochemistrycell biologyhumanmouse

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A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation
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A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Molecular recognition reagents are essential for biological research, with antibodies being the most common.
  • Antibodies can exhibit issues like poor characterization, stability, and batch variability.
  • Alternative binding proteins are needed as complementary tools for various applications.

Purpose of the Study:

  • To evaluate Affimer proteins as research reagents for molecular and cellular applications.
  • To demonstrate the utility of Affimer proteins in diverse biological contexts.
  • To highlight Affimer proteins as complementary tools to antibodies.

Main Methods:

  • Selection of Affimer proteins against 12 diverse molecular targets.
  • Application of Affimers in protein function modulation (in vitro and in vivo).
  • Utilisation of Affimers for tumor antigen labeling in mouse models and advanced microscopy.

Main Results:

  • Successful selection of Affimers against a range of molecular targets.
  • Demonstrated efficacy of Affimers in modulating protein function.
  • Effective use of Affimers in in vivo imaging and super-resolution microscopy.

Conclusions:

  • Affimer proteins are effective and versatile research reagents.
  • Affimers serve as valuable complements to traditional antibody reagents.
  • Affimer technology expands the toolkit for molecular and cell biology research.