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Specific and selective probes for Staphylococcus aureus from phage-displayed random peptide libraries.

Laura M De Plano1, Santina Carnazza1, Grazia M L Messina2

  • 1Department of Chemical Sciences, Biological, Pharmaceutical and Environmental, University of Messina, Viale F. Stagnod'Alcontres 31, 98166 Messina, Italy.

Colloids and Surfaces. B, Biointerfaces
|June 28, 2017
PubMed
Summary

Researchers developed a phage-based biosensor for rapid Staphylococcus aureus detection. This system specifically binds to S. aureus, offering a promising tool for early diagnosis and clinical applications.

Keywords:
Biosensor selective probesFunctionalization of mica surfacePathogen detectionPhage displayStaphylococcus aureus

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Area of Science:

  • Microbiology
  • Biotechnology
  • Nanotechnology

Background:

  • Staphylococcus aureus is a significant human pathogen responsible for healthcare-associated and community-associated infections.
  • Early and accurate diagnosis of S. aureus infections is critical for preventing disease progression and severe complications.

Purpose of the Study:

  • To isolate and characterize a phage clone with specific binding capabilities to Staphylococcus aureus.
  • To develop a novel biosensing strategy for the rapid and selective detection of S. aureus.

Main Methods:

  • Phage display library screening to identify S. aureus-specific phage clones.
  • Enzyme-linked immune-sorbent assay (ELISA) and Western blot analysis to confirm phage-bacteria binding and identify target proteins.
  • Atomic Force Microscopy (AFM) for phage immobilization and surface characterization.
  • Fluorescent microscopy to evaluate real-time binding performance of the biosensor.

Main Results:

  • A phage clone, St.au9IVS5, displaying a peptide (RVRSAPSSS) with specific binding to S. aureus was identified.
  • The phage peptide demonstrated selective binding to a 78KDa protein on the S. aureus cell surface.
  • The developed biosensor immobilized on mica showed specific binding to S. aureus, detecting approximately 50% of cells within 15 minutes and 90% within one hour.
  • The biosensor exhibited selectivity, enabling the identification of clinical S. aureus isolates among other bacterial species.

Conclusions:

  • A novel phage-based biosensing strategy for specific and rapid detection of Staphylococcus aureus has been successfully developed.
  • The biosensor's efficiency and specificity show potential for application in clinical diagnostics, particularly as a lab-on-chip (LOC) device.
  • This technology offers a promising avenue for developing cost-effective diagnostic tools for bacterial agents, especially in resource-limited settings.