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Updated: Feb 27, 2026

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Published on: February 1, 2014
Rapid identification of ST131 Escherichia coli by a novel multiplex real-time allelic discrimination assay
Patrice François1, Eve-Julie Bonetti2, Carolina Fankhauser3
1Genomic Research Laboratory, University of Geneva Hospitals and Faculty of Medicine, Geneva, Switzerland; Bacteriology Laboratory, University of Geneva Hospitals and Faculty of Medicine, Geneva, Switzerland.
Abstract:
Escherichia coli sequence type 131 is increasingly described in severe hospital infections. We developed a rapid real-time allelic discrimination assay for the rapid identification of E. coli ST131 isolates. This rapid assay represents an affordable alternative to sequence-based strategies before completing characterization of potentially highly virulent isolates of E. coli.
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