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High-throughput format for the phenotyping of fungi on solid substrates
David Cánovas1,2, Lena Studt3,4, Ana T Marcos5,6
1Division of Microbial Genetics and Pathogen Interaction, Department of Applied Genetics and Cell Biology, BOKU University of Natural Resources and Life Science, Campus Tulln, Tulln/Donau, Austria. davidc@us.es.
Scientific Reports
|June 29, 2017
Summary
This study introduces a new high-throughput platform for analyzing filamentous fungi growth and gene expression on solid media, mimicking natural conditions. The system offers novel insights into fungal development and the regulation of secondary metabolite production.
Area of Science:
- Mycology
- Molecular Biology
- Biotechnology
Background:
- Filamentous fungi are typically studied in liquid cultures, which do not reflect their natural solid-surface growth.
- Understanding fungal growth and gene expression in natural conditions is crucial for various applications.
Purpose of the Study:
- To develop and validate a high-throughput photometric platform for analyzing fungal growth and reporter gene expression on solid media.
- To investigate fungal growth characteristics and secondary metabolite gene regulation under diverse conditions.
Main Methods:
- A multiplexing platform with high-throughput photometric continuous reading was developed.
- The system allows parallel quantification of hyphal growth and reporter gene expression directly on solid medium.
- Experiments were conducted on Aspergillus and Fusarium species exposed to varying nutrients, stress, and epigenetic modifiers.
Main Results:
- The platform successfully quantified fungal growth and secondary metabolite reporter gene expression (GFP-based) in real-time on solid media.
- Novel insights into fungal growth patterns were obtained.
- Metabolic and time-dependent regulation of secondary metabolite gene expression was elucidated.
Conclusions:
- The developed platform effectively mimics natural fungal growth conditions on solid media.
- This method provides valuable insights into fungal physiology and the regulation of secondary metabolism.
- The system is applicable to both saprophytic and phytopathogenic filamentous fungi.
