Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

5.3K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

4.8K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.8K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

4.9K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.9K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

5.7K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.7K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

6.5K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
6.5K
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

11.6K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
11.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Phellinus linteus</i> polysaccharides inhibit castration-resistant prostate cancer progression possibly via dual modulation of PIK3R1 and PGC-1α: a preclinical evaluation.

American journal of cancer research·2026
Same author

Fu Zheng Xiao Yu San Jie Decoction affects the proliferation of renal cell carcinoma via regulating <i>E2F5</i> gene.

Translational andrology and urology·2026
Same author

Relationships between intervertebral disc degeneration and lysyl oxidase expression in human nucleus pulposus.

Biomedical reports·2025
Same author

MiR-501-3p/SPC24 axis affects cell proliferation, migration, invasion, apoptosis, and prognosis in renal cell carcinoma.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2025
Same author

Retraction notice to "MiR-23a-3p acts as an oncogene and potential prognostic biomarker by targeting PNRC2 in RCC" Biomed. Pharmacother. 110(2019) 656-666.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2024
Same author

[Retracted] Oncogenic miR-23a-5p is associated with cellular function in RCC.

Molecular medicine reports·2024

Related Experiment Video

Updated: Feb 27, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.1K

Oncogenic miR-23a-5p is associated with cellular function in RCC.

Jing Quan1, Lu Jin1, Xiang Pan1

  • 1Department of Urology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.

Molecular Medicine Reports
|June 29, 2017
PubMed
Summary

MicroRNAs (miRs) play a role in tumor development. This study found miR-23a-5p acts as an oncogene in renal cell carcinoma (RCC), promoting cancer cell growth and spread, suggesting it as a potential therapeutic target.

More Related Videos

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

11.3K
Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
05:36

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

Published on: February 8, 2020

9.5K

Related Experiment Videos

Last Updated: Feb 27, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.1K
miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

11.3K
Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
05:36

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

Published on: February 8, 2020

9.5K

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNAs (miRs) are increasingly recognized for their roles in tumorigenesis.
  • miR-23a-5p has been identified as an oncogene in various diseases.
  • The specific role of miR-23a-5p in renal cell carcinoma (RCC) was previously unclear.

Purpose of the Study:

  • To investigate the expression and function of miR-23a-5p in renal cell carcinoma (RCC).
  • To determine if miR-23a-5p acts as an oncogene in RCC development.
  • To explore miR-23a-5p as a potential therapeutic target for RCC.

Main Methods:

  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for miR-23a-5p expression analysis.
  • Cell Counting Kit-8 (CCK-8), MTT, wound scratch, and Transwell assays for proliferation, migration, and invasion.
  • Flow cytometry to assess apoptosis.
  • Experiments conducted on RCC tissue samples and cell lines (786O, ACHN, Caki-1).

Main Results:

  • miR-23a-5p expression was significantly upregulated in RCC tissues and cell lines compared to normal controls.
  • Upregulation of miR-23a-5p promoted RCC cell proliferation, migration, and invasion.
  • Downregulation of miR-23a-5p inhibited these oncogenic processes and promoted cell viability.
  • miR-23a-5p was found to inhibit RCC cell apoptosis.

Conclusions:

  • miR-23a-5p functions as an oncogene in the occurrence and development of renal cell carcinoma.
  • miR-23a-5p represents a potential novel therapeutic target for RCC treatment.