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Nondisjunction is the failure of homologous chromosomes or sister chromatids to separate correctly and move to the opposite poles of the cells. This produces daughter cells with abnormal chromosome numbers. Nondisjunction is common during anaphase I or anaphase II of meiosis. Mutations in synaptonemal complex proteins that attach homologous chromosomes increase the chances of nondisjunction in anaphase I of meiosis I. In contrast, mutations in topoisomerases and condensins that hold...

Nondisjunction

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Related Experiment Video

Updated: Feb 27, 2026

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

To compare aneuploidy rates between ICSI and IVF Cases.

L Sahin¹, M Bozkurt², H Şahin³

¹Department in Obstetrics and Gynecology, Kafkas University School of Medicine, Kars, Turkey.

Nigerian Journal of Clinical Practice

|June 29, 2017

Summary

Intracytoplasmic sperm injection (ICSI) does not significantly increase aneuploidy compared to in vitro fertilization (IVF). This study found similar rates of chromosome abnormalities between the two assisted reproductive technologies.

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Published on: September 7, 2021

Author Spotlight: Exploring the Long-Term Health Impacts of Intracytoplasmic Sperm Injection on Offspring

Author Spotlight: Exploring the Long-Term Health Impacts of Intracytoplasmic Sperm Injection on Offspring

Published on: May 17, 2024

Area of Science:

Reproductive biology
Human genetics
Assisted reproductive technologies

Background:

Intracytoplasmic sperm injection (ICSI) is a key treatment for male infertility.
Potential risks of ICSI include asynchronous sperm decondensation and damage to the meiotic spindle, potentially leading to aneuploidy.
A comparison with in vitro fertilization (IVF) is warranted to assess aneuploidy risks.

Purpose of the Study:

To investigate whether ICSI is associated with a higher incidence of aneuploidy compared to IVF.
To analyze chromosome abnormalities in embryos resulting from both ICSI and IVF procedures.

Main Methods:

A retrospective study involving 36 individuals undergoing either IVF or ICSI.
Fluorescent in-situ hybridization (FISH) was used to detect abnormalities in seven specific chromosome pairs (X, Y, 13, 16, 18, 21, 22).
Statistical analysis included Chi-square tests and odds ratios with confidence intervals.

Main Results:

A total of 57 embryos from IVF and 183 embryos from ICSI were analyzed.
The abnormality rates were 65% for IVF embryos and 69.9% for ICSI embryos.
No statistically significant difference in aneuploidy rates was observed between ICSI and IVF (odds ratio: 0.948, 95% CI: 0.678-1.324).

Conclusions:

ICSI does not significantly increase the incidence of aneuploidy when compared to IVF.
FISH analysis of seven chromosome pairs supports the conclusion of comparable aneuploidy rates between the two methods.