To compare aneuploidy rates between ICSI and IVF Cases

Area of Science:

• Reproductive biology
• Human genetics
• Assisted reproductive technologies

Background:

• Intracytoplasmic sperm injection (ICSI) is a key treatment for male infertility.
• Potential risks of ICSI include asynchronous sperm decondensation and damage to the meiotic spindle, potentially leading to aneuploidy.
• A comparison with in vitro fertilization (IVF) is warranted to assess aneuploidy risks.

Purpose of the Study:

• To investigate whether ICSI is associated with a higher incidence of aneuploidy compared to IVF.
• To analyze chromosome abnormalities in embryos resulting from both ICSI and IVF procedures.

Main Methods:

• A retrospective study involving 36 individuals undergoing either IVF or ICSI.
• Fluorescent in-situ hybridization (FISH) was used to detect abnormalities in seven specific chromosome pairs (X, Y, 13, 16, 18, 21, 22).
• Statistical analysis included Chi-square tests and odds ratios with confidence intervals.

Main Results:

• A total of 57 embryos from IVF and 183 embryos from ICSI were analyzed.
• The abnormality rates were 65% for IVF embryos and 69.9% for ICSI embryos.
• No statistically significant difference in aneuploidy rates was observed between ICSI and IVF (odds ratio: 0.948, 95% CI: 0.678-1.324).

Conclusions:

• ICSI does not significantly increase the incidence of aneuploidy when compared to IVF.
• FISH analysis of seven chromosome pairs supports the conclusion of comparable aneuploidy rates between the two methods.