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Molecular Strain Typing of Clinical Isolates, Trichophyton rubrum using Non Transcribed Spacer (NTS) Region as a

Vijayakumar Ramaraj1, Rajyoganandh S Vijayaraman1, Elangovan Elavarashi2

  • 1Scholar, Department of Microbiology, Sri Ramachandra Medical College and Research Institute, SRU, Chennai, Tamil Nadu, India.

Journal of Clinical and Diagnostic Research : JCDR
|June 30, 2017
PubMed
Abstract

Insights

This study identified nine distinct strains of Trichophyton rubrum using Non Transcribed Spacer (NTS) region analysis. This molecular marker effectively differentiates clinical isolates, aiding in understanding fungal infection epidemiology and transmission.

Area of Science:

  • Mycology
  • Molecular Biology
  • Epidemiology

Background:

  • Dermatophytes, including Trichophyton, Microsporum, and Epidermophyton, cause superficial fungal infections.
  • Trichophyton rubrum is a common cause of chronic dermatophytosis, with reinfections increasing treatment costs.
  • Accurate strain discrimination is crucial for understanding the clinical and epidemiological significance of T. rubrum genetic diversity.

Purpose of the Study:

  • To perform strain typing of clinical Trichophyton rubrum isolates.
  • To utilize the Non Transcribed Spacer (NTS) region of ribosomal DNA as a molecular marker for strain discrimination.

Main Methods:

  • Seventy clinical T. rubrum isolates were identified using conventional phenotypic methods.
  • Polymerase Chain Reaction (PCR) was employed to target two subrepeat elements (TRS-1 and TRS-2) within the NTS region.
  • Analysis focused on identifying strain-specific polymorphisms in the NTS region.

Main Results:

  • Strain-specific polymorphism was successfully observed in both TRS-1 and TRS-2 subrepeat loci.
  • Combining data from both subrepeat elements, a total of nine distinct T. rubrum strains were identified.
  • The NTS region proved effective in differentiating clinical isolates.

Conclusions:

  • Amplification of the NTS region is a robust method for discriminating T. rubrum clinical isolates.
  • This molecular typing approach can be adapted for epidemiological and population-based studies of T. rubrum infections.
  • The findings facilitate future research into the epidemiology of T. rubrum.
Keywords:
Sub repeat elementsTRS -1TRS-2

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