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WciG O-Acetyltransferase Functionality Differentiates Pneumococcal Serotypes 35C and 42.

K Aaron Geno1, C Allen Bush2, Mengnan Wang3

  • 1Department of Medicine, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA.

Journal of Clinical Microbiology
|June 30, 2017
PubMed
Summary
This summary is machine-generated.

Streptococcus pneumoniae serotypes 35C and 42 differ in O-acetylation due to a functional WciG enzyme in 35C and a deletion in 42. This explains their distinct capsular polysaccharide structures and serological properties.

Keywords:
O-acetyltransferaseStreptococcus pneumoniaebiochemical structurecapsular polysaccharidegenetic basisserological profileserotyping

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Area of Science:

  • Microbiology
  • Structural Biology
  • Genetics

Background:

  • Streptococcus pneumoniae uses capsular polysaccharides (CPSs) for protection.
  • The cps locus governs CPS synthesis in most pneumococcal serotypes.
  • CPS structures and their genetic basis are not fully elucidated for all serotypes.

Purpose of the Study:

  • To clarify discrepancies in the reported structure and genetic locus of Streptococcus pneumoniae serotype 35C.
  • To differentiate serotype 35C from the closely related serotype 42.
  • To investigate the role of O-acetylation in pneumococcal CPS structure and serotyping.

Main Methods:

  • Analysis of clinical and reference isolates of Streptococcus pneumoniae serotypes 35C and 42.
  • Serological characterization and genetic sequencing of the cps locus.
  • Purification and structural elucidation of CPSs.

Main Results:

  • The O-acetyltransferase WciG is functional in serotype 35C but nonfunctional in serotype 42 due to a deletion in wciG.
  • Serotype 35C CPS exhibits O-acetylation at specific positions of galactofuranose, while serotype 42 shows limited O-acetylation.
  • These structural differences correlate with the functionality of WciG and serological reactions.

Conclusions:

  • The functional status of WciG is a key determinant of CPS structure and O-acetylation in Streptococcus pneumoniae serotypes 35C and 42.
  • This study provides a comprehensive understanding of the genetic, structural, and serological basis differentiating these two serotypes.
  • Findings contribute to the characterization of pneumococcal capsule diversity and its implications.