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Extra Large G-Protein Interactome Reveals Multiple Stress Response Function and Partner-Dependent XLG Subcellular
Ying Liang1,2, Yajun Gao1, Alan M Jones2,3
1College of Natural Resources and Environment, Northwest A&F UniversityXianyang, China.
Frontiers in Plant Science
|June 30, 2017
Summary
Arabidopsis extra-large G proteins (XLG) interact with numerous proteins, including transcription factors. These XLG-protein interactions are crucial for stress responses and gene expression, influencing plant development.
Area of Science:
- Plant molecular biology
- Cell signaling
- Genetics
Background:
- Arabidopsis extra-large G proteins (XLG1-3) are atypical Gα subunits involved in root morphology, stress response, and development.
- Downstream targets of XLG proteins in stress pathways are largely unknown.
Purpose of the Study:
- To identify proteins interacting with XLG proteins.
- To elucidate the role of XLG proteins in stress response pathways.
Main Methods:
- Yeast two-hybrid complementation screen using XLG protein baits.
- Analysis of protein interactions in glucose-treated seedlings, roots, and cultured Arabidopsis cells.
- Gene co-expression analysis and Gene Ontology enrichment.
Main Results:
- Seventy-two XLG protein interactors were identified, with over 60% confirmed by in vivo interaction.
- Over 70% of identified interactors showed positive co-expression with their XLG partners.
- Gene Ontology enrichment indicated roles in stress responses, identifying transcription factors SZF1 and SZF2 as key partners.
Conclusions:
- XLG proteins interact with a diverse set of proteins, including transcription factors, suggesting a role in regulating gene expression.
- XLG proteins are essential for the full expression of NaCl-induced genes, such as SZF1 and SZF2.
- Subcellular localization of XLG proteins is dependent on their interacting partners, indicating dynamic regulation.
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