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Adoptive Transfer of Lung Antigen Presenting Cells

Xiaofeng Zhou1, Bethany B Moore1

  • 1Department of Internal Medicine, Pulmonary and Critical Care Medicine Division, University of Michigan, Ann Arbor, Michigan, USA.

Bio-Protocol
|June 30, 2017
PubMed

Our protocol describes adoptive transfer of antigen presenting cells (APCs) isolated from the lungs by enzymatic digestion and magnetic enrichment. This protocol can be used to study APC functions and trafficking.

Keywords:
Adoptive transferDendritic cellsEnzymatic digestionIntravenous injectionLung antigen presenting cellsMacrophagesMagnetic cell isolation

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