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Adoptive Transfer of Lung Antigen Presenting Cells
Xiaofeng Zhou1, Bethany B Moore1
1Department of Internal Medicine, Pulmonary and Critical Care Medicine Division, University of Michigan, Ann Arbor, Michigan, USA.
Bio-Protocol
|June 30, 2017
Summary
This study details a method for isolating lung antigen-presenting cells (APCs) for adoptive transfer. The protocol enables the study of APC functions and cellular trafficking in vivo.
Area of Science:
- Immunology
- Cell Biology
- Respiratory Medicine
Background:
- Antigen-presenting cells (APCs) are crucial for initiating adaptive immune responses.
- Understanding APC function and trafficking in the lungs is vital for respiratory health and disease research.
Purpose of the Study:
- To describe a novel protocol for the isolation and adoptive transfer of lung APCs.
- To provide a method for investigating APC functions and trafficking within the pulmonary microenvironment.
Main Methods:
- Isolation of APCs from lung tissue using enzymatic digestion.
- Magnetic enrichment techniques for purifying APC populations.
- Adoptive transfer of isolated APCs for functional studies.
Main Results:
- Successful isolation of viable APCs from the lung.
- Demonstration of the protocol's utility in studying APC function.
- Establishment of a method to track APC trafficking.
Conclusions:
- The described protocol offers a reliable method for lung APC isolation and adoptive transfer.
- This technique facilitates in-depth studies of pulmonary APCs and their roles in immune responses.
