Oxidative lipidomics: applications in critical care

Area of Science:

• Biochemistry
• Pathophysiology
• Analytical Chemistry

Background:

• Lipid peroxidation is integral to critical illness pathophysiology.
• Oxidative lipidomics advances understanding of lipid oxidation in health and disease.

Purpose of the Study:

• Review recent achievements in oxidative lipidomics.
• Highlight contributions to understanding critical illness.

Main Methods:

• Liquid chromatography tandem mass spectrometry (LC-MS/MS) for oxidative lipidomics.
• Identification of enzymatically generated lipid oxidation products.

Main Results:

• Focusing on end products misses early lipid peroxidation events.
• Identified diverse lipid mediators in injury, inflammation, and recovery.
• These mediators are crucial in sepsis and head trauma.

Conclusions:

• Lipid oxidation products, enzymatically or chemically formed, regulate cellular signaling.
• Oxidative lipidomics quantifies products, elucidates mechanisms, and reveals synergistic functions.
• Advances inform future critical illness interventions.