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Conjugation systems of IncT plasmids.

D E Bradley, J Whelan

    Journal of General Microbiology
    |October 1, 1985
    PubMed
    Summary

    Temperature affects IncT plasmid conjugation and pilus synthesis. At 37°C, pili are short, limiting conjugation to surfaces, unlike at 30°C where longer pili enable surface and liquid conjugation.

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    Area of Science:

    • Molecular Biology
    • Microbiology
    • Genetics

    Background:

    • IncT plasmids are conjugative elements in bacteria.
    • Pilus synthesis and function are crucial for bacterial conjugation.
    • Temperature sensitivity can affect plasmid conjugation efficiency.

    Purpose of the Study:

    • To compare pilus synthesis and function of four IncT plasmids at different temperatures.
    • To investigate the impact of temperature on IncT plasmid conjugation systems.
    • To understand the role of pilus length in conjugation efficiency.

    Main Methods:

    • Comparative analysis of four IncT plasmids (Rts1, R402, R394, pIN25).
    • Assessment of pilus synthesis and length at 30°C and 37°C.
    • Evaluation of conjugation efficiency in liquid and on surfaces at different temperatures.

    Main Results:

    • The Rts1 plasmid exhibited distinct characteristics compared to other IncT plasmids.
    • Conjugation systems functioned on surfaces but not in liquid at 37°C.
    • Long conjugative pili were synthesized at 30°C, while shorter pili (approx. 200 nm) were produced at 37°C.
    • Long pili facilitated surface and liquid conjugation at 30°C, converting surface-obligatory systems.

    Conclusions:

    • Temperature significantly influences IncT plasmid pilus length and conjugation strategy.
    • Pilus length is a key factor determining the ability of IncT plasmids to conjugate in liquid media.
    • The findings provide insights into the regulation of conjugation by temperature-sensitive mechanisms.

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