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Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
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Persistent luminescence tomography for small animal imaging.

Xu Cao1, Yuzhu Gong1, Yang Li1

  • 1Engineering Research Center of Molecular and Neuro Imaging of the Ministry of Education & School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710071, China.

Biomedical Optics Express
|July 1, 2017
PubMed
Summary

Persistent luminescence tomography (PLT) offers high-quality 3D imaging for small animals by overcoming autofluorescence limitations. This novel method enables clear visualization of deep tissue probes without excitation light interference.

Keywords:
(170.3010) Image reconstruction techniques(170.3880) Medical and biological imaging(170.6960) Tomography

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Area of Science:

  • Biomedical Optics
  • Preclinical Imaging
  • Optical Tomography

Background:

  • Fluorescence imaging in preclinical studies is hindered by low signal-to-noise ratio (SNR) due to tissue autofluorescence and excitation light.
  • Persistent luminescence (PeL) imaging emerges as a promising alternative, circumventing autofluorescence and excitation light bleed-through.
  • Existing methods lack the capability for deep-tissue 3D visualization of PeL probes.

Purpose of the Study:

  • To introduce a novel tomographic imaging method based on persistent luminescence (PLT).
  • To enable three-dimensional (3D) reconstruction of persistent luminescence probe distributions deep within small animals.
  • To address the limitations of conventional fluorescence imaging in terms of SNR and interference.

Main Methods:

  • Development of a persistent luminescence tomography (PLT) system.
  • Utilizing persistent luminescence signals that remain for hours post-excitation.
  • Phantom and live mouse experiments for validation.

Main Results:

  • PLT successfully reconstructs high-quality 3D tomographic images.
  • The method effectively avoids interference from autofluorescence and excitation light.
  • Demonstrated feasibility of deep-tissue imaging in small animal models.

Conclusions:

  • Persistent luminescence tomography (PLT) is a viable technique for high-SNR, 3D in vivo imaging.
  • PLT overcomes key limitations of traditional fluorescence imaging in preclinical research.
  • This method holds significant potential for advancing deep-tissue optical imaging applications.