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Related Experiment Video

Updated: Feb 27, 2026

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

Lenslet array tunable snapshot imaging spectrometer (LATIS) for hyperspectral fluorescence microscopy.

Jason G Dwight¹, Tomasz S Tkaczyk^1,2

¹Rice University, Department of Bioengineering, 6500 Main St., Houston, TX 77030, USA.

Biomedical Optics Express

|July 1, 2017

Summary

A new snapshot hyperspectral imaging system, the lenslet array tunable snapshot imaging spectrometer (LATIS), offers tunable spectral resolution for advanced fluorescence microscopy. This innovation enables faster, high-resolution imaging with fewer artifacts.

Keywords:

(110.4234) Multispectral and hyperspectral imaging (170.2520) Fluorescence microscopy (170.3880) Medical and biological imaging

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Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals

Published on: April 14, 2020

Area of Science:

Optics and Imaging
Spectroscopy
Biomedical Engineering

Background:

Snapshot hyperspectral imaging enhances speed and pixel dwell time in fluorescence microscopy.
Existing systems often have fixed spectral parameters, limiting flexibility.
There is a need for adaptable hyperspectral imaging solutions.

Purpose of the Study:

To introduce a novel tunable snapshot hyperspectral imaging system called LATIS.
To demonstrate the system's capability for adjustable spectral resolution and large field-of-view imaging.
To evaluate the performance of LATIS in high-resolution fluorescence microscopy.

Main Methods:

Development of the lenslet array tunable snapshot imaging spectrometer (LATIS).
Characterization of LATIS's tunable spectral resolution (22.66 nm to 13.94 nm) across 485-660 nm.
Implementation of a fixed LATIS configuration for large field-of-view imaging (200x200x27 datacube).

Main Results:

LATIS demonstrated tunable spectral resolution, achieving an average of 13.94 nm.
A large field-of-view configuration provided a 200x200 datacube.
High-resolution fluorescence imaging was achieved with exposure times under 1 second and minimal artifacts.

Conclusions:

LATIS offers a flexible and powerful solution for snapshot hyperspectral imaging.
The tunable nature of LATIS enhances its applicability in fluorescence microscopy.
The system provides high-quality imaging with rapid acquisition and reduced artifacts.