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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Feb 27, 2026

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium

Published on: June 24, 2014

Identification of pterygium-related mRNA expression profiling by microarray analysis.

J Liu¹, X Ding², L Yuan¹

¹Department of Ophthalmology, Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.

Eye (London, England)

|July 1, 2017

Summary

This study identified numerous differentially expressed messenger RNAs (mRNAs) in pterygium tissues, revealing their potential role in this ocular surface disease. These findings suggest mRNAs could be new therapeutic targets for pterygium treatment.

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Last Updated: Feb 27, 2026

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium

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Published on: March 20, 2009

Area of Science:

Ophthalmology
Molecular Biology
Genomics

Background:

Pterygium is a degenerative ocular surface disease with proliferative characteristics, sometimes leading to visual impairment.
Its pathogenesis remains incompletely understood despite similarities to tumorigenesis.
Previous research explored long non-coding RNAs (lncRNAs) in pterygium.

Purpose of the Study:

To investigate the potential roles of messenger RNAs (mRNAs) in pterygium pathogenesis.
To identify differentially expressed mRNAs in pterygium tissues.

Main Methods:

Construction of pterygium-related mRNA libraries using microarray analysis.
Validation of selected deregulated mRNAs via quantitative real-time PCR (qRT-PCR) in 10 patients.

Main Results:

Identification of 1485 upregulated and 2978 downregulated mRNAs in pterygium tissues compared to normal conjunctiva.
qRT-PCR confirmed the differential expression of four upregulated and two downregulated mRNAs.

Conclusions:

The study reveals distinct mRNA expression profiles in pterygium.
These differentially expressed mRNAs represent potential novel molecular targets for pterygium therapy.