Related Experiment Video
Updated: Feb 27, 2026
08:40
Photothrombotic Ischemia: A Minimally Invasive and Reproducible Photochemical Cortical Lesion Model for Mouse Stroke Studies
Published on: June 9, 2013
37.1K
[The brain after ischemic stroke: a clinical/histological study]
S P Sergeeva1, A A Savin1, L V Shishkina1
1Sechenov First Moscow State Medical University, Moscow, Russia, Evdokimov Moscow State University of Medicine and Dentistry, Moscow, Russia, Burdenko Scientific Research Neurosurgery, Moscow, Russia.
Summary
Ischemic stroke causes significant brain tissue changes, affecting neurons and glial cells even in areas distant from the initial damage. These alterations may contribute to the brain's capacity for neuroplasticity.
Area of Science:
- Neuropathology
- Neuroscience
- Histology
Background:
- Ischemic stroke leads to neuronal death and brain tissue damage.
- Understanding the extent and nature of histological changes is crucial for prognosis and treatment.
Purpose of the Study:
- To analyze the histological features of brain tissue changes following ischemic stroke.
- To investigate the distribution and severity of these changes in relation to the necrotic core.
Main Methods:
- Autopsy brain tissue samples from 9 ischemic stroke patients (3-7 days post-stroke) were analyzed.
- Samples were taken from areas adjacent to, distant from, and contralateral to the necrotic core.
- Histological staining (Nissl, H&E) and immunohistochemistry (p53, NSE, GFAP) were employed.
Main Results:
- Observed decreased neuron and glial cell counts, altered cell structure, and spatial redistribution.
- Changes in artery walls and impaired regional blood flow were noted.
- Increased NSE reactivity, p53-positive neurons, and reduced neuron-astrocyte distances were found in remote and contralateral areas.
Conclusions:
- Ischemic stroke induces severe histological changes throughout the brain, not limited to the infarct core.
- These widespread changes, varying in severity, represent a substrate for potential neuroplasticity.
- The findings highlight the complex neuropathological consequences of ischemic stroke.
