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Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
Published on: October 21, 2016
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Visualization and Quantification of IgG Antibody Adsorbed at the Cellulose-Liquid Interface
Vikram Singh Raghuwanshi1, Jielong Su1, Christopher J Garvey2
1Bioresource Processing Research Institute of Australia (BioPRIA), Department of Chemical Engineering, Monash University , Clayton, Victoria 3800, Australia.
Biomacromolecules
|July 1, 2017
Summary
A new method uses deuterated cellulose to visualize and quantify antibody adsorption for eco-friendly biodiagnostics. This technique enhances contrast, enabling clear imaging of antibody layers on cellulose surfaces.
Area of Science:
- Biomolecular science
- Materials science
- Surface science
Background:
- Quantifying biomolecule adsorption at interfaces is crucial for biodiagnostics.
- Developing eco-friendly biodiagnostic tools requires precise interface analysis.
- Current methods face challenges in visualizing adsorbed molecules on cellulose.
Purpose of the Study:
- To develop a novel methodology for visualizing and quantifying antibody adsorption onto cellulose.
- To enhance scattering contrast at the cellulose-liquid interface using deuteration.
- To establish a foundation for improved eco-friendly biodiagnostic platforms.
Main Methods:
- Deuterated cellulose (DC) synthesis using heavy water and deuterated glycerol.
- Preparation of hydrogenated cellulose (HC) and DC thin films on silicon substrates.
- X-ray reflectivity (XR) for film characterization.
- Neutron reflectivity (NR) for interface analysis and adsorption visualization.
- Quartz crystal microbalance with dissipation monitoring (QCM-D) for quantification.
Main Results:
- Successful synthesis of homogeneous and smooth HC and DC films.
- Neutron reflectivity revealed significant swelling of cellulose films in solution.
- Clear visualization of Immunoglobulin G (IgG) antibody adsorption on DC films due to enhanced contrast.
- Quantification of adsorbed IgG layer thickness (127 ± 5 Å) and formation of a partial monolayer.
- Demonstrated significant difference in scattering length density (SLD) between DC and IgG in D2O.
Conclusions:
- Deuteration of cellulose significantly enhances scattering contrast for NR analysis.
- The developed method enables precise visualization and quantification of antibody adsorption.
- This approach offers a promising pathway for developing advanced eco-friendly biodiagnostics.
