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Updated: Feb 27, 2026

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

Published on: August 22, 2016

Crowding in Cellular Environments at an Atomistic Level from Computer Simulations.

Michael Feig^1,2, Isseki Yu^3,4, Po-Hung Wang³

¹Department of Biochemistry and Molecular Biology, Michigan State University , East Lansing, Michigan, United States.

The Journal of Physical Chemistry. B

|July 1, 2017

Summary

This summary is machine-generated.

Cellular crowding significantly impacts biomolecular structure and dynamics. Computer simulations reveal how crowding can alter protein energy landscapes, potentially destabilizing native states and affecting molecular motion.

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Area of Science:

Biophysics
Computational Biology
Structural Biology

Background:

The influence of crowded biological environments on biomolecular behavior is poorly understood.
Cellular interiors present complex conditions including macromolecular crowding and altered solvent properties.

Purpose of the Study:

To investigate the effects of crowding on the structure, dynamics, and function of peptides and proteins.
To provide insights into how cellular environments remodel biomolecular energy landscapes.

Main Methods:

Utilized computer simulations of atomistic models for concentrated peptide and protein systems.
Examined systems at varying levels of complexity to understand crowding effects.

Main Results:

Crowding, weak interactions, and altered solvent properties significantly remodel biomolecular energy landscapes.
Observed potential for native state destabilization in crowded environments.
Crowding affects both conformational dynamics and diffusional properties of macromolecules.

Conclusions:

Computer simulations offer valuable insights into the effects of biological crowding.
Crowding plays a crucial role in modulating biomolecular properties within cellular environments.
Findings align with and provide context for relevant experimental observations.