Related Experiment Video
Updated: Feb 27, 2026

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
Published on: June 21, 2018
Rapid analysis of benzalkonium chloride using paper spray mass spectrometry
Jingjing Liu1, Wenjie Deng2, Muqian Yu2
1Key Laboratory of Phytochemical R&D of Hunan Province and Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, China; College of Chemistry and Chemical Engineering, University of South China, Hengyang, 421001, China.
Abstract:
A paper spray mass spectrometry (PS-MS) method for rapid and reliable analysis of benzalkonium chloride (BAC) in compound eye drops and body surface disinfectant was developed. The sample was dropped onto triangular filter paper, and high voltage (3.5kV) was applied to form an electrospray. This method can provide the composition of benzalkonium chloride in samples without pretreatment, solvent or chromatographic separation, and the analysis time is only 10s. The primary homologues C12-BAC, C14-BAC and C16-BAC of benzalkonium chloride were quantitatively analyzed using PS-MS. Samples were subjected to simple dilution and quantified using the internal standard method. Ion trap mass spectrometry was scanned using SIM mode. The linear ranges of C12-BAC, C14-BAC and C16-BAC were 1-100μgmL-1; the linear regression coefficients were 0.998-0.999; the detection limits (LODs) were 0.1μgmL-1; the limit of quantifications (LOQs) was <1μgmL-1, and the method validation indicated that the method precision and accuracy were good. Compared with HPLC-UV methods, there was no significant difference in the quantitative determination of the actual samples, but the analysis time for PS-MS is shorter (2min). In addition, reagent consumption in PS-MS is small, and no chromatographic separation is needed, suggesting that PS-MS is especially suitable for high-throughput analysis.
More Related Videos
07:53Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry
Published on: March 1, 2020
04:17Analysis of Organochlorine Pesticides in a Soil Sample by a Modified QuEChERS Approach Using Ammonium Formate
Published on: January 20, 2023
Related Concept Videos
Electrospray Ionization (ESI) Mass Spectrometry
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Mass Spectrometry: Alkyl Halide Fragmentation
Mass Spectrometry: Aromatic Compound Fragmentation
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
NMR Spectroscopy of Benzene Derivatives
Chemical Ionization (CI) Mass Spectrometry