Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

RelB/ILF2-GM-CSF loop sustains tumor-MDSC communication in prostate cancer immunosuppression.

Cell death & disease·2026

Same author

A humanized engineered heart tissue platform for cardiotoxicity assessment.

Stem cell reports·2026

Same author

Cellular polarity pilots breast cancer progression and immunosuppression.

Oncogene·2025

Same author

Functional inversion of circadian regulator REV-ERBα leads to tumorigenic gene reprogramming.

Proceedings of the National Academy of Sciences of the United States of America·2024

Same author

Revisiting the role of mesenchymal stromal cells in cancer initiation, metastasis and immunosuppression.

Experimental hematology & oncology·2024

Same author

Post-death Vesicles of Senescent Bone Marrow Mesenchymal Stromal Polyploids Promote Macrophage Aging and Breast Cancer.

bioRxiv : the preprint server for biology·2024

Search research articles

Related Experiment Video

Updated: Feb 27, 2026

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective.

Maria Navas-Moreno¹, Majid Mehrpouyan², Tatyana Chernenko²

¹University of California-Davis, Center for Biophotonics, Sacramento, 95817, USA.

Scientific Reports

|July 2, 2017

Summary

Surface enhanced Raman scattering (SERS) nanoparticles offer stable, multiplexed biological labeling. However, their rapid uptake and intracellular trafficking in live cells limit their use for monitoring dynamic surface biomarker expression.

More Related Videos

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

Author Spotlight: Advancements and Applications in Nanoparticle Synthesis Through Laser Ablation in Liquids

Author Spotlight: Advancements and Applications in Nanoparticle Synthesis Through Laser Ablation in Liquids

Published on: June 16, 2023

Related Experiment Videos

Last Updated: Feb 27, 2026

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

Author Spotlight: Advancements and Applications in Nanoparticle Synthesis Through Laser Ablation in Liquids

Author Spotlight: Advancements and Applications in Nanoparticle Synthesis Through Laser Ablation in Liquids

Published on: June 16, 2023

Area of Science:

Nanotechnology
Biomedical Engineering
Cell Biology

Background:

Surface-enhanced Raman scattering (SERS) nanoparticles are promising for biological labeling due to photostability and multiplexing.
Nanoparticle characteristics (size, shape, surface) and protein corona formation impact cell interactions.
Antibody multivalency also affects nanoparticle-antibody conjugate performance.

Purpose of the Study:

To evaluate SERS nanoparticles as probes for live cell imaging.
To investigate nanoparticle-cell interactions and trafficking in live cells.
To compare SERS nanoparticle labeling with fluorescent probes for cell dynamics.

Main Methods:

Utilized SERS nanoparticles targeting HER2 and CD44 biomarkers on breast cancer cells.
Labeled both fixed and live cells to assess specificity and dynamics.
Compared SERS nanoparticle behavior with fluorescent antibody conjugates in live cells.

Main Results:

Achieved specific labeling of fixed cells with SERS nanoparticles, comparable to fluorescent labels.
Observed rapid nanoparticle uptake and intracellular compartmentalization in live cells.
Demonstrated that nanoparticle internalization contrasts sharply with fluorescent probe behavior.

Conclusions:

SERS nanoparticles can achieve specific labeling in fixed cells.
Nanoparticle internalization and intracellular trafficking impede live cell surface monitoring.
The dynamics of SERS nanoparticle-cell interactions must be considered for live imaging applications.