Related Experiment Video
Updated: Feb 27, 2026

Quantifying Yersinia pseudotuberculosis Type III Secretion System Activity Following Iron Starvation and Anaerobic Growth
Published on: May 31, 2024
Energy Requirements for Protein Secretion via the Flagellar Type III Secretion System
1Helmholtz Centre for Infection Research, Inhoffenstr. 7, Braunschweig, 38124, Germany. marc.erhardt@helmholtz-hzi.de.
Abstract:
Protein transport across the cytoplasmic membrane is coupled to energy derived from adenosine triphosphate hydrolysis or the protein motive force (pmf). A sophisticated, multi-component type III secretion system exports substrate proteins of both the bacterial flagellum and virulence-associated injectisome system of many Gram-negative pathogens. The type-III secretion system is primarily a pmf-driven protein exporter. Here, I describe methods to investigate the export of substrate proteins into the culture supernatant under conditions that manipulate the pmf.
More Related Videos
09:12Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
Published on: March 10, 2020
13:11Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
Published on: February 10, 2023
Related Concept Videos
Gram-negative Bacterial Protein Secretion Systems
Bacterial Translocation and Protein Secretion
Overview of Secretory Vesicles
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Flagella and Motility in Bacteria
Stringent Response in E. coli
Mechanical Protein Functions