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Updated: Feb 27, 2026

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation
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A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

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Identification of Effectors: Precipitation of Supernatant Material

Nicolas Flaugnatti1, Laure Journet2

  • 1Laboratoire d'Ingénierie des Systèmes Macromoléculaires (LISM, UMR7255), Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRS, 31 Chemin Joseph Aiguier, 13402, Marseille Cedex 20, France.

Methods in Molecular Biology (Clifton, N.J.)
|July 2, 2017
PubMed

Keywords:
SecretomeSupernatantTCA precipitation

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