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Related Experiment Video

Updated: Feb 27, 2026

A Microfluidic-based Hydrodynamic Trap for Single Particles
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Controllable picoliter pipetting using hydrophobic microfluidic valves.

M Zhang1, J Huang1, X Qian1

  • 1Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.

The Review of Scientific Instruments
|July 3, 2017
PubMed
Summary

This study introduces a microfluidic pipetting technique for precise picoliter liquid handling. The novel method uses hydrophobic valves to accurately dispense volumes under 100 picoliters for various applications.

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Area of Science:

  • Microfluidics
  • Nanotechnology
  • Biotechnology

Background:

  • Accurate liquid handling at the picoliter scale is crucial for many scientific applications.
  • Existing methods for picoliter dispensing can be complex and expensive.

Purpose of the Study:

  • To develop a novel microfluidic technique for precise picoliter pipetting.
  • To demonstrate the controlled dispensing of sub-nanoliter liquid volumes.

Main Methods:

  • Utilized microfluidic channels with patterned hydrophobic self-assembled monolayer films.
  • Employed T-shaped channel junctions and hydrophobic patches to create pressure-controlled valves and liquid traps.
  • Controlled liquid volume by adjusting the distance of the hydrophobic patch from the T-junction.

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Last Updated: Feb 27, 2026

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Main Results:

  • Successfully developed a picoliter pipetting technique.
  • Achieved precise separation and ejection of liquid volumes less than 100 picoliters.
  • Demonstrated accurate volume control by modifying channel geometry.

Conclusions:

  • The developed microfluidic device offers a precise and potentially cost-effective method for picoliter liquid handling.
  • This technique has significant potential for sample dispensing in biological, medical, and chemical fields.