Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Lean management for improving hospital waiting times-Case study of a Vietnamese public/general hospital emergency department.

The International journal of health planning and management·2021

Same author

<i>In-vitro</i> study of monocytic THP-1 leukemia cell membrane elasticity with a single-cell microfluidic-assisted optical trapping system.

Biomedical optics express·2020

Same author

Neural Analyses Validate and Emphasize the Role of Progesterone Receptor in Breast Cancer Progression and Prognosis.

Anticancer research·2016

Same author

Real-time evaluation of breast self-examination using computer vision.

International journal of biomedical imaging·2014

Same author

Logic-centered architecture for ubiquitous health monitoring.

IEEE journal of biomedical and health informatics·2014

Same author

Computer vision-based breast self-examination stroke position and palpation pressure level classification using artificial neural networks and wavelet transforms.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2013

Search research articles

Related Experiment Video

Updated: Feb 27, 2026

Microfluidic Device for the Separation of Non-Metastatic MCF-7 and Non-Tumor MCF-10A Breast Cancer Cells Using AC Dielectrophoresis

Microfluidic Device for the Separation of Non-Metastatic MCF-7 and Non-Tumor MCF-10A Breast Cancer Cells Using AC Dielectrophoresis

Published on: August 11, 2022

Effects of Pulsed Electromagnetic Fields on Breast Cancer Cell Line MCF 7 Using Absorption Spectroscopy.

Dominic Z Alcantara¹, Ian Jerry S Soliman¹, Romeric F Pobre¹

¹Optics and Instrumentation Physics Laboratory, Physics Department, De La Salle University, Manila, Philippines.

Anticancer Research

|July 3, 2017

Summary

Pulsed electromagnetic fields (PEMF) targeting specific gene resonant frequencies significantly affected MCF-7 breast cancer cells in vitro. Different exposure durations were required for RICTOR, PPARG, NBN, and CHEK2 genes.

Keywords:

Breast cancer PrestoBlueT™gene mutation pulsed electromagnetic fields spectroscopy

More Related Videos

Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer

Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer

Published on: May 21, 2019

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

Related Experiment Videos

Last Updated: Feb 27, 2026

Microfluidic Device for the Separation of Non-Metastatic MCF-7 and Non-Tumor MCF-10A Breast Cancer Cells Using AC Dielectrophoresis

Microfluidic Device for the Separation of Non-Metastatic MCF-7 and Non-Tumor MCF-10A Breast Cancer Cells Using AC Dielectrophoresis

Published on: August 11, 2022

Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer

Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer

Published on: May 21, 2019

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

Area of Science:

Biophysics
Cell Biology
Electromagnetism

Background:

The DNA in MCF-7 breast cancer cells possesses electric dipole properties, making it susceptible to electromagnetic fields.
Pulsed electromagnetic fields (PEMF) offer a potential non-invasive method for biological modulation.
Understanding cellular responses to specific electromagnetic frequencies is crucial for therapeutic development.

Purpose of the Study:

To investigate the in vitro effects of PEMF, modulated by audio resonant frequencies of specific genes, on MCF-7 breast cancer cells.
To determine the viability of MCF-7 cells under varying PEMF treatment conditions using absorption spectroscopy.
To identify specific gene resonant frequencies and exposure durations that elicit a significant cellular response.

Main Methods:

Utilized absorption spectroscopy with PrestoBlue™ Cell Viability Reagent to quantify MCF-7 cell viability.
Applied PEMF with a 3.3 MHz carrier frequency, modulated by resonant frequencies derived from RICTOR, PPARG, NBN, and CHEK2 genes.
Measured changes in cell absorption values to assess treatment efficacy and employed t-tests for statistical significance.

Main Results:

PEMF treatment at the resonant frequencies of RICTOR and PPARG genes showed significant effects on MCF-7 cells after 10 minutes of exposure.
Significant effects were observed for NBN gene resonant frequency after 15 minutes and CHEK2 gene resonant frequency after 5 minutes of PEMF exposure.
Absorption spectroscopy data indicated differential cellular responses based on gene-specific resonant frequencies and exposure times.

Conclusions:

PEMF, when modulated by specific gene resonant frequencies, can significantly impact MCF-7 breast cancer cell viability in vitro.
Exposure duration is a critical factor, with varying optimal times identified for each targeted gene (RICTOR, PPARG, NBN, CHEK2).
This study highlights the potential of frequency-specific PEMF as a targeted approach in breast cancer research.