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Updated: Feb 27, 2026
Establishment and Characterization of Three Afatinib-resistant Lung Adenocarcinoma PC-9 Cell Lines Developed with Increasing Doses of Afatinib
Published on: June 26, 2019
The Effect of Afatinib Treatment in Non-small Cell Lung Cancer Cells
Joanna Pancewicz-Wojtkiewicz1, Pawel Leszek Bernatowicz2
1Department of Histology and Embryology, Medical University of Bialystok, Bialystok, Poland jpancewicz@ymail.com.
Background:
Non-small cell lung cancer (NSCLC) is a very aggressive and histologically heterogeneous type of lung cancer. The main problem in the treatment of NSCLC is chemoresistance and metastasis. Compared to other malignant tumors, many molecular mechanisms are dysfunctional in NSCLC. Epidermal growth factor receptor (EGFR) is one of the most frequently mutated genes in NSCLC.
Materials And Methods:
We investigated the effect of afatinib the against A549 NSCLC cell line, because it is active against mutated EGFR. Moreover, we aimed to investigate the mRNA level of HSPA5 (one of the heat shock proteins that can contribute in the down-regulation of the EGF-signaling pathway) before and after afatinib treatment.
Results:
Afatinib treatment induced apoptosis and decreased levels of HSPA5 mRNA in cancer cells.
Conclusion:
Advanced analysis, might be helpful in understanding the mechanisms of relations between tyrosine kinase inhibition of EGFR and HSPA5 in lung cancer cells.
Insights
Afatinib treatment induced apoptosis in non-small cell lung cancer (NSCLC) cells. This study also observed a decrease in HSPA5 mRNA levels following afatinib administration in NSCLC.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Non-small cell lung cancer (NSCLC) is an aggressive cancer characterized by chemoresistance and metastasis.
- Dysfunctional molecular mechanisms are common in NSCLC, with frequent mutations in the epidermal growth factor receptor (EGFR).
Purpose of the Study:
- To investigate the effects of afatinib on the A549 NSCLC cell line.
- To examine the impact of afatinib on the mRNA levels of heat shock protein HSPA5, which can down-regulate the EGF-signaling pathway.
Main Methods:
- Treatment of A549 NSCLC cells with afatinib.
- Analysis of HSPA5 mRNA levels before and after afatinib treatment.
Main Results:
- Afatinib treatment led to induced apoptosis in NSCLC cells.
- A significant decrease in HSPA5 mRNA levels was observed post-afatinib treatment.
Conclusions:
- Afatinib demonstrates efficacy in inducing apoptosis in NSCLC cells.
- Further research into the relationship between EGFR tyrosine kinase inhibition and HSPA5 in lung cancer is warranted.
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