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Tris buffer effects on melanophore aggregating responses.

M A Visconti, A M Castrucci

    Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology
    |January 1, 1985
    PubMed
    Summary
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    Tris (hydroxymethyl)-aminomethane) may affect fish melanophore responses by influencing neurotransmitter availability. This study reveals Tris

    Area of Science:

    • Pharmacology
    • Cell Biology
    • Neuroscience

    Background:

    • Tris (hydroxymethyl)-aminomethane) is a common buffer in biological research.
    • Its effects on cellular responses, particularly in pigment cells, are not well understood.
    • Melanophores are key cells in regulating skin coloration in fish.

    Purpose of the Study:

    • To investigate the adverse effects of Tris buffer on fish melanophore responses.
    • To compare melanophore reactions in Tris-buffered versus bicarbonate-buffered solutions.
    • To elucidate the potential mechanisms of Tris action on melanophore signaling.

    Main Methods:

    • Comparison of melanophore responses in Tris-buffered and bicarbonate-buffered solutions.
    • Dose-response curve analysis for norepinephrine, methoxamine, and potassium chloride.

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  • Assessment of aggregation response to synthetic melanin-concentrating hormone (MCH).

    • Main Results:

    • Tris significantly shifted the norepinephrine dose-response curve leftward.
    • Methoxamine dose-response curves were unaffected by Tris.
    • Potassium chloride dose-response curve showed a leftward shift in Tris buffer.
    • Tris did not affect MCH-induced aggregation.

    Conclusions:

    • Tris may influence melanophore responses by affecting presynaptic mechanisms or catechol-O-methyltransferase (COMT) activity.
    • This suggests an increased availability of neurotransmitters at melanophore receptors in the presence of Tris.
    • Further research is needed to fully understand Tris' impact on neuroendocrine signaling in fish.