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JNK pathway mediates curcumin-induced apoptosis and autophagy in osteosarcoma MG63 cells

Yaowu Zhang1, Pingbo Chen1, Hangang Hong1

  • 1Department of Orthopedic Surgery, Traditional Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830001, P.R. China.

Experimental and Therapeutic Medicine
|July 5, 2017
PubMed

Insights

Curcumin induces apoptosis and autophagy in osteosarcoma cells. Autophagy may promote resistance to curcumin-induced apoptosis, offering insights for cancer treatment strategies.

Area of Science:

  • Oncology
  • Cell Biology
  • Biochemistry

Background:

  • Human osteosarcoma is a prevalent bone cancer in children and adolescents.
  • Curcumin is known to induce apoptosis in osteosarcoma MG63 cells via the mitochondrial pathway.
  • The interplay between curcumin-induced autophagy and apoptosis in osteosarcoma remains unclear.

Purpose of the Study:

  • To investigate if curcumin induces autophagy in osteosarcoma cells.
  • To elucidate the interaction between apoptosis and autophagy in response to curcumin.
  • To explore the role of the c-Jun N-terminal kinase (JNK) signaling pathway.

Main Methods:

  • Treatment of MG63 osteosarcoma cells with curcumin.
  • Assessment of apoptosis and autophagy induction.
  • Inhibition of apoptosis and autophagy using specific inhibitors (SP600125, 3-methyladenine).
  • Analysis of the c-Jun N-terminal kinase (JNK) signaling pathway.

Main Results:

  • Curcumin significantly induced both apoptosis and autophagy in MG63 cells.
  • Inhibiting apoptosis enhanced curcumin-induced autophagy, mediated by JNK pathway activation.
  • Inhibiting autophagy increased curcumin-induced apoptosis.
  • Autophagy appears to play a role in resistance to curcumin-induced apoptosis.

Conclusions:

  • Curcumin elicits both apoptosis and autophagy in osteosarcoma cells.
  • Autophagy may confer resistance to curcumin-induced apoptosis, potentially through JNK signaling.
  • Understanding this interaction is crucial for developing effective curcumin-based therapeutic strategies for osteosarcoma.
Keywords:
apoptosisautophagycurcuminosteosarcoma

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