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Geometric morphometrics in mosquitoes: What has been measured?
Camila Lorenz1, Fabio Almeida2, Fernanda Almeida-Lopes2
1Instituto Butantan, Av. Vital Brazil 1500, Butantã, São Paulo, SP CEP 0553-900, Brazil; Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes 1374, Butantã, São Paulo, SP CEP 05508-900, Brazil.
Summary
Geometric morphometrics (GM) effectively distinguishes mosquito groups by analyzing variations within and between species, including sexual dimorphism. Careful data processing is crucial for reliable results in mosquito research.
Area of Science:
- Zoology
- Morphometrics
Background:
- Geometric morphometrics (GM) is rapidly advancing.
- GM techniques are increasingly applied to zoological problems, especially concerning epidemiologically relevant mosquitoes.
Purpose of the Study:
- To introduce geometric morphometric techniques.
- To review the application of GM in mosquito (Culicidae) research.
- To forecast future directions in the field.
Main Methods:
- Review of selected mosquito groups where GM techniques have been applied.
- Analysis of GM's capability in discriminating among mosquito groups based on various factors.
- Discussion of data processing considerations for reliable biometrical analysis.
Main Results:
- GM successfully discriminated among mosquito groups in most reviewed studies.
- Discrimination was most effective when considering inter- and intra-species differences, sexual dimorphism, treatments, and laboratory strains.
- Caution is advised regarding data processing for allometry, asymmetry, scale removal, and wing staining.
Conclusions:
- Geometric morphometrics is a powerful tool for mosquito research, aiding in species identification and understanding variations.
- Standardized data processing protocols are essential for robust GM analyses.
- Development of image databases holds promise for enhancing culicid species identification.

